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Tetracycline exposure shifted microbial communities and enriched antibiotic resistance genes in the aerobic granular sludge
Abstract The aerobic granular sludge with larger size and more compact spherical structure generally shows excellent performance in antibiotic removal, yet little is known about the long-term effect of environmentally-relevant concentration (μg/L) of antibiotics on the proliferation of antibiotic resistance genes (ARGs) and microbial community in aerobic granules. Herein, a sequencing batch reactor (SBR) was set up with dosing different concentrations (0–500 μg/L) of tetracycline to investigate its influences on microbial communities and ARG levels in aerobic granular sludge. Results show that the bioreactor could effectively remove chemical oxygen demand (COD), nitrogen, and tetracycline during the long-term operation. The quantitative polymerase chain reaction (qPCR) analysis shows that tetracycline at μg/L level could greatly enhance the absolute and relative abundances of tetA, sulII, and bla TEM-1 in the effluent and aerobic granules, indicating tetracycline could serve as a selection pressure on the development of ARGs corresponding to different types of antibiotics in aerobic granules. Pearson's correlation analysis also implies that sulII and bla TEM-1 were correlated strongly with tetA. Moreover, the presence of tetracycline altered the microbial communities and diversity of the effluent and aerobic granules in the bioreactor. These findings would advance our understanding of the proliferation and development of ARGs in aerobic granules under tetracycline pressure and serve as a foundation to guide the application of aerobic granular sludge for treatment of antibiotic-containing wastewater.
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Highlights Tetracycline did not inhibit the performance of bioreactor in pollutant removal. Tetracycline altered microbial community and diversity of aerobic granular sludge. Long-term pressure of tetracycline could co-enrich tetA, sulII, and bla TEM-1. sulII and bla TEM-1 were strongly correlated with tetA in aerobic granular sludge.
Tetracycline exposure shifted microbial communities and enriched antibiotic resistance genes in the aerobic granular sludge
Abstract The aerobic granular sludge with larger size and more compact spherical structure generally shows excellent performance in antibiotic removal, yet little is known about the long-term effect of environmentally-relevant concentration (μg/L) of antibiotics on the proliferation of antibiotic resistance genes (ARGs) and microbial community in aerobic granules. Herein, a sequencing batch reactor (SBR) was set up with dosing different concentrations (0–500 μg/L) of tetracycline to investigate its influences on microbial communities and ARG levels in aerobic granular sludge. Results show that the bioreactor could effectively remove chemical oxygen demand (COD), nitrogen, and tetracycline during the long-term operation. The quantitative polymerase chain reaction (qPCR) analysis shows that tetracycline at μg/L level could greatly enhance the absolute and relative abundances of tetA, sulII, and bla TEM-1 in the effluent and aerobic granules, indicating tetracycline could serve as a selection pressure on the development of ARGs corresponding to different types of antibiotics in aerobic granules. Pearson's correlation analysis also implies that sulII and bla TEM-1 were correlated strongly with tetA. Moreover, the presence of tetracycline altered the microbial communities and diversity of the effluent and aerobic granules in the bioreactor. These findings would advance our understanding of the proliferation and development of ARGs in aerobic granules under tetracycline pressure and serve as a foundation to guide the application of aerobic granular sludge for treatment of antibiotic-containing wastewater.
Graphical abstract Display Omitted
Highlights Tetracycline did not inhibit the performance of bioreactor in pollutant removal. Tetracycline altered microbial community and diversity of aerobic granular sludge. Long-term pressure of tetracycline could co-enrich tetA, sulII, and bla TEM-1. sulII and bla TEM-1 were strongly correlated with tetA in aerobic granular sludge.
Tetracycline exposure shifted microbial communities and enriched antibiotic resistance genes in the aerobic granular sludge
Zhang, Ming-Qi (author) / Yuan, Li (author) / Li, Zheng-Hao (author) / Zhang, Han-Chao (author) / Sheng, Guo-Ping (author)
2019-06-05
Article (Journal)
Electronic Resource
English
Resistance to salt stresses by aerobic granular sludge: sludge property and microbial community
| Springer Verlag | 2024