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Histidine modified montmorillonite: Laccase immobilization and application to flow injection analysis of phenols
Abstract The preparation, characterization and application of histidine (His) modified montmorillonite (His-Mt) in electrochemical biosensor fabrication were reported. His was used as a modifier to enhance immobilization capability of Mt for the biomolecules. Mt modifications were followed by X-ray diffraction, Fourier transform infrared spectroscopy, zeta potential and thermal gravimetry analysis. After a successful immobilization of Laccase enzyme on the His-Mt matrix, covered on a glassy carbon electrode, application in catechol biosensing was investigated in detail. Optimization, analytical characteristics, repeatability, and operational stability studies were evaluated using both batch and flow injections analysis configurations.
Graphical abstract Display Omitted
Highlights Na+-montmorillonite was intercalated with Histidine amino acid. A phenol biosensor consist of immobilized laccase using histidine-modified montmorillonite Optimized biosensor for best response and electrochemical behavior in addition to analytical characteristics
Histidine modified montmorillonite: Laccase immobilization and application to flow injection analysis of phenols
Abstract The preparation, characterization and application of histidine (His) modified montmorillonite (His-Mt) in electrochemical biosensor fabrication were reported. His was used as a modifier to enhance immobilization capability of Mt for the biomolecules. Mt modifications were followed by X-ray diffraction, Fourier transform infrared spectroscopy, zeta potential and thermal gravimetry analysis. After a successful immobilization of Laccase enzyme on the His-Mt matrix, covered on a glassy carbon electrode, application in catechol biosensing was investigated in detail. Optimization, analytical characteristics, repeatability, and operational stability studies were evaluated using both batch and flow injections analysis configurations.
Graphical abstract Display Omitted
Highlights Na+-montmorillonite was intercalated with Histidine amino acid. A phenol biosensor consist of immobilized laccase using histidine-modified montmorillonite Optimized biosensor for best response and electrochemical behavior in addition to analytical characteristics
Histidine modified montmorillonite: Laccase immobilization and application to flow injection analysis of phenols
Songurtekin, Didem (author) / Yalcinkaya, Esra Evrim (author) / Ag, Didem (author) / Seleci, Muharrem (author) / Demirkol, Dilek Odaci (author) / Timur, Suna (author)
Applied Clay Science ; 86 ; 64-69
2013-10-12
6 pages
Article (Journal)
Electronic Resource
English
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