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Biocementation of sand by Sporosarcina pasteurii strain and technical-grade cementation reagents through surface percolation treatment method
Highlights Low-cost growth medium was used to cultivate Sporosarcina pasteurii prepared in deionized water and tap water. Soil biocementation treatment was performed through surface percolation method. Analytical-grade cementation reagents can be replaced with technical-grade cementation reagents. Utilizing technical-grade reagents for sand biocementation resulted in 47 to 51-fold cost reduction.
Abstract The use of microbially induced carbonate precipitation (MICP) to produce biocementitious material for soil stabilization has emerged in recent decades as a sustainable alternative approach to conventional methods. However, the use of standard analytical-grade reagents for various MICP studies makes this technology very expensive and unsuitable for field-scale consideration. In this present study, the feasibility of using commercially available and inexpensive technical-grade reagents for the cultivation of ureolytic bacteria and enhancement of soil stabilization was investigated. Low-cost growth media prepared in deionized water and tap water were used to cultivate Sporosarcina pasteurii as a replacement to standard laboratory-grade media. Biocement treatment was carried out on sand columns using different concentrations (0.25–1.0 M) of technical-grade and analytical-grade cementation solutions via surface percolation method. After 92 h of treatment, the columns were cured for 3 weeks at room temperature (26 ± 2 °C) before analysing their respective surface strengths, CaCO3 content, pH of effluents and sand microscopic structures. The results indicated that the growth of bacteria in low-cost cultivation medium was similar to that observed in the standard cultivation medium. Surface strengths and CaCO3 contents of the consolidated samples were in the ranges of 11448.00 ± 69.00–4826.00 ± 00 kPa and 5.56 ± 1.15–33.24 ± 0.59%, respectively. Overall, the obtained results of the current study encourage future MICP studies to utilize commercially available technical-grade reagents for economical MICP field-scale trials.
Biocementation of sand by Sporosarcina pasteurii strain and technical-grade cementation reagents through surface percolation treatment method
Highlights Low-cost growth medium was used to cultivate Sporosarcina pasteurii prepared in deionized water and tap water. Soil biocementation treatment was performed through surface percolation method. Analytical-grade cementation reagents can be replaced with technical-grade cementation reagents. Utilizing technical-grade reagents for sand biocementation resulted in 47 to 51-fold cost reduction.
Abstract The use of microbially induced carbonate precipitation (MICP) to produce biocementitious material for soil stabilization has emerged in recent decades as a sustainable alternative approach to conventional methods. However, the use of standard analytical-grade reagents for various MICP studies makes this technology very expensive and unsuitable for field-scale consideration. In this present study, the feasibility of using commercially available and inexpensive technical-grade reagents for the cultivation of ureolytic bacteria and enhancement of soil stabilization was investigated. Low-cost growth media prepared in deionized water and tap water were used to cultivate Sporosarcina pasteurii as a replacement to standard laboratory-grade media. Biocement treatment was carried out on sand columns using different concentrations (0.25–1.0 M) of technical-grade and analytical-grade cementation solutions via surface percolation method. After 92 h of treatment, the columns were cured for 3 weeks at room temperature (26 ± 2 °C) before analysing their respective surface strengths, CaCO3 content, pH of effluents and sand microscopic structures. The results indicated that the growth of bacteria in low-cost cultivation medium was similar to that observed in the standard cultivation medium. Surface strengths and CaCO3 contents of the consolidated samples were in the ranges of 11448.00 ± 69.00–4826.00 ± 00 kPa and 5.56 ± 1.15–33.24 ± 0.59%, respectively. Overall, the obtained results of the current study encourage future MICP studies to utilize commercially available technical-grade reagents for economical MICP field-scale trials.
Biocementation of sand by Sporosarcina pasteurii strain and technical-grade cementation reagents through surface percolation treatment method
Omoregie, Armstrong I. (author) / Palombo, Enzo A. (author) / Ong, Dominic E.L. (author) / Nissom, Peter M. (author)
2019-08-28
Article (Journal)
Electronic Resource
English
Biological Clogging in Tangshan Sand Columns under Salt Water Intrusion by Sporosarcina pasteurii
| British Library Conference Proceedings | 2011
| Taylor & Francis Verlag | 2024