A platform for research: civil engineering, architecture and urbanism
A platform for research: civil engineering, architecture and urbanism
Polystyrene nanoplastics induce profound metabolic shift in human cells as revealed by integrated proteomic and metabolomic analysis
Graphical abstract Display Omitted
Highlights A multi-omic analysis was performed to study the impact of nanoplastics on human cells. Alteration of the molecular landscape induced by PSNPs was systematically depicted. PSNPs induced metabolic reprograming. PSNPs affected cellular metabolism through inhibiting mTORC1 signaling.
Abstract Nanoplastics (NPLs) are widespread in our environment. However, their impacts on human health and precise toxicity mechanisms remain poorly understood. Here we studied the internalization, release, and cytotoxicity of polystyrene nanoplastics (PSNPs) using the renal tubular epithelial cell line HKC and human derived liver cell line HL-7702. We also employed an integrated proteomic and metabolomic approach to investigate the potential biological effects of PSNPs on HKC cells. The abundances of 4770 proteins and 100 metabolites were quantified, with 785 proteins and 17 metabolites detected with altered levels in response to PSNPs. Most of the differential proteins and metabolites were enriched in a variety of metabolic pathways, for example, glycolysis, citrate cycle, oxidative phosphorylation, and amino acid metabolism, suggesting the potential effects of NPLs on global cellular metabolism shift in human cells. The altered energy metabolism induced by PSNPs was further confirmed by a Seahorse analysis. Moreover, lysosomal distribution study and western blotting showed that mTORC1 signaling, a central regulator of cellular metabolism, was inhibited upon nanoplastic exposure, likely serving as the link between lysosome dysfunction and metabolic defects. Taken together, our findings systematically mapped the key molecular changes induced by PSNPs in human cells and provide comprehensive biological insights for the risk estimation of NPLs contamination.
Polystyrene nanoplastics induce profound metabolic shift in human cells as revealed by integrated proteomic and metabolomic analysis
Graphical abstract Display Omitted
Highlights A multi-omic analysis was performed to study the impact of nanoplastics on human cells. Alteration of the molecular landscape induced by PSNPs was systematically depicted. PSNPs induced metabolic reprograming. PSNPs affected cellular metabolism through inhibiting mTORC1 signaling.
Abstract Nanoplastics (NPLs) are widespread in our environment. However, their impacts on human health and precise toxicity mechanisms remain poorly understood. Here we studied the internalization, release, and cytotoxicity of polystyrene nanoplastics (PSNPs) using the renal tubular epithelial cell line HKC and human derived liver cell line HL-7702. We also employed an integrated proteomic and metabolomic approach to investigate the potential biological effects of PSNPs on HKC cells. The abundances of 4770 proteins and 100 metabolites were quantified, with 785 proteins and 17 metabolites detected with altered levels in response to PSNPs. Most of the differential proteins and metabolites were enriched in a variety of metabolic pathways, for example, glycolysis, citrate cycle, oxidative phosphorylation, and amino acid metabolism, suggesting the potential effects of NPLs on global cellular metabolism shift in human cells. The altered energy metabolism induced by PSNPs was further confirmed by a Seahorse analysis. Moreover, lysosomal distribution study and western blotting showed that mTORC1 signaling, a central regulator of cellular metabolism, was inhibited upon nanoplastic exposure, likely serving as the link between lysosome dysfunction and metabolic defects. Taken together, our findings systematically mapped the key molecular changes induced by PSNPs in human cells and provide comprehensive biological insights for the risk estimation of NPLs contamination.
Polystyrene nanoplastics induce profound metabolic shift in human cells as revealed by integrated proteomic and metabolomic analysis
Wang, Hua (author) / Shi, Xuelian (author) / Gao, Yan (author) / Zhang, Xiangyang (author) / Zhao, Hongzhi (author) / Wang, Lei (author) / Zhang, Xu (author) / Chen, Ruibing (author)
2022-06-13
Article (Journal)
Electronic Resource
English
Nanoplastics , Proteomics , Metabolomics , mTORC1 , Toxicity
Sorption Behavior of Trace Organic Chemicals on Carboxylated Polystyrene Nanoplastics
| American Chemical Society | 2024