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Aminofunctionalized LAPONITE® as a versatile hybrid material for chlorhexidine digluconate incorporation: Cytotoxicity and antimicrobial activities
Abstract Aminofunctionalized LAPONITE® RD was employed for efficient chlorhexidine digluconate (CHX-DG) incorporation. The structure and properties of the unloaded (Lap-APTES) and loaded (Lap-APTES:CHX-DG) hybrids were investigated by powder X-ray diffraction (PXRD), thermal analysis, small angle X-ray scattering (SAXS), and infrared absorption spectroscopy (FTIR). A typical adsorption experiment was conducted to evaluate how the parameters contact time and concentration affected CHX-DG incorporation into the clay and showed that this compound had high affinity for Lap-APTES, adsorption equilibrium was reached at 90 min, with CHX-DG incorporation of 1402.7 mg/g. FTIR analysis confirm the interaction by hydrogen bonds between the CHX-DG and APTES amine groups and the laponite OH groups, resulting in materials with potential antibacterial properties for controlled drug release. Even at low concentrations (0.042 mg/g), Lap-APTES:CHX-DG was effective against S. pyogenes. The Lap, Lap-APTES, and Lap-APTES:CHX-DG cytotoxicity to GM07492A cells (human fibroblasts) was investigated by the XTT colorimetric assay, which revealed that CHX-DG incorporation into Lap-APTES reduced drug cytotoxicity. Drug release experiments demonstrated that a maximum of 10% (m/m) CHX-DG was released within 24 h and confirmed the higher affinity between Lap-APTES and CHX-DG.
Graphical abstract Display Omitted
Highlights LAPONITE®RD with amine groups are loaded with chlorhexidine by adsorption The interaction on surface and interlayer space of solid occurs via hydrogen bonds. In vitro drug delivery reveal that 10% of chlorhexidine were released after 24h Incorporation of chlorhexidine into Lap-APTES reduced drug cytotoxicity Antibacterial results shown Lap-APTES:CHX-DG was effective against S. pyogenes
Aminofunctionalized LAPONITE® as a versatile hybrid material for chlorhexidine digluconate incorporation: Cytotoxicity and antimicrobial activities
Abstract Aminofunctionalized LAPONITE® RD was employed for efficient chlorhexidine digluconate (CHX-DG) incorporation. The structure and properties of the unloaded (Lap-APTES) and loaded (Lap-APTES:CHX-DG) hybrids were investigated by powder X-ray diffraction (PXRD), thermal analysis, small angle X-ray scattering (SAXS), and infrared absorption spectroscopy (FTIR). A typical adsorption experiment was conducted to evaluate how the parameters contact time and concentration affected CHX-DG incorporation into the clay and showed that this compound had high affinity for Lap-APTES, adsorption equilibrium was reached at 90 min, with CHX-DG incorporation of 1402.7 mg/g. FTIR analysis confirm the interaction by hydrogen bonds between the CHX-DG and APTES amine groups and the laponite OH groups, resulting in materials with potential antibacterial properties for controlled drug release. Even at low concentrations (0.042 mg/g), Lap-APTES:CHX-DG was effective against S. pyogenes. The Lap, Lap-APTES, and Lap-APTES:CHX-DG cytotoxicity to GM07492A cells (human fibroblasts) was investigated by the XTT colorimetric assay, which revealed that CHX-DG incorporation into Lap-APTES reduced drug cytotoxicity. Drug release experiments demonstrated that a maximum of 10% (m/m) CHX-DG was released within 24 h and confirmed the higher affinity between Lap-APTES and CHX-DG.
Graphical abstract Display Omitted
Highlights LAPONITE®RD with amine groups are loaded with chlorhexidine by adsorption The interaction on surface and interlayer space of solid occurs via hydrogen bonds. In vitro drug delivery reveal that 10% of chlorhexidine were released after 24h Incorporation of chlorhexidine into Lap-APTES reduced drug cytotoxicity Antibacterial results shown Lap-APTES:CHX-DG was effective against S. pyogenes
Aminofunctionalized LAPONITE® as a versatile hybrid material for chlorhexidine digluconate incorporation: Cytotoxicity and antimicrobial activities
Peraro, Gustavo R. (author) / Donzelli, Eduardo H. (author) / Oliveira, Pollyanna F. (author) / Tavares, Denise Crispim (author) / Gomes Martins, Carlos H. (author) / Molina, Eduardo F. (author) / de Faria, Emerson H. (author)
Applied Clay Science ; 195
2020-06-10
Article (Journal)
Electronic Resource
English
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