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A platform for research: civil engineering, architecture and urbanism
High-volume sampler for size-selective sampling of bioaerosols including viruses
https://orcid.org/0000-0002-8365-5760
https://orcid.org/0000-0002-7419-021X
https://orcid.org/0000-0001-8197-0401
https://orcid.org/0000-0002-6005-2991
https://orcid.org/0000-0003-1804-5373
Abstract Owing to the recent global spread of the new coronavirus SARS-CoV-2, the development of technology to effectively detect viruses in crowded public places is urgently needed. In this study, a three-stage high-volume bioaerosol sampler was developed for the size-selective sampling of bioaerosols through the suction of air at a high flow rate of 1000 L/min. In stage 1, an omnidirectional inlet cyclone separator that can draw air from all directions was applied to collect bioaerosols larger than 10 μm in the collection fluid. In stage 2, an axial flow cyclone separator was used to collect bioaerosols sized between 2.5 and 10 μm in the collection fluid. In stage 3, bioaerosols smaller than 2.5 μm were collected on a filter and extracted in a solution through an elution process using a sodium phosphate buffer. To simulate the suspension of bioparticles including viruses that are attached to other particles in the atmosphere, the aerosol samples were prepared by coagulating aerosolized bacteriophages with Arizona test dust. Then, the coagulated particles were collected for 30 min using the developed bioaerosol sampler, and the samples collected in each stage were analyzed via polymerase chain reaction (PCR) method. The PCR analysis results confirmed that the high-volume bioaerosol sampler enables size-selective bioaerosol sampling even at a high airflow rate of 1000 L/min. The developed high-volume bioaerosol sampler will be useful in detecting viruses through PCR analysis because it can collect bioaerosols within a specific size range.
Highlights We designed 3-stage high-volume bioaerosol sampler with size-selective sampling. The sampler draws air at a high flow rate of 1000 L/min. We simulated the suspension of bioparticles attached to other particles. We analyzed the bioaerosol samples collected in each stage via PCR method.
High-volume sampler for size-selective sampling of bioaerosols including viruses
https://orcid.org/0000-0002-8365-5760
https://orcid.org/0000-0002-7419-021X
https://orcid.org/0000-0001-8197-0401
https://orcid.org/0000-0002-6005-2991
https://orcid.org/0000-0003-1804-5373
Abstract Owing to the recent global spread of the new coronavirus SARS-CoV-2, the development of technology to effectively detect viruses in crowded public places is urgently needed. In this study, a three-stage high-volume bioaerosol sampler was developed for the size-selective sampling of bioaerosols through the suction of air at a high flow rate of 1000 L/min. In stage 1, an omnidirectional inlet cyclone separator that can draw air from all directions was applied to collect bioaerosols larger than 10 μm in the collection fluid. In stage 2, an axial flow cyclone separator was used to collect bioaerosols sized between 2.5 and 10 μm in the collection fluid. In stage 3, bioaerosols smaller than 2.5 μm were collected on a filter and extracted in a solution through an elution process using a sodium phosphate buffer. To simulate the suspension of bioparticles including viruses that are attached to other particles in the atmosphere, the aerosol samples were prepared by coagulating aerosolized bacteriophages with Arizona test dust. Then, the coagulated particles were collected for 30 min using the developed bioaerosol sampler, and the samples collected in each stage were analyzed via polymerase chain reaction (PCR) method. The PCR analysis results confirmed that the high-volume bioaerosol sampler enables size-selective bioaerosol sampling even at a high airflow rate of 1000 L/min. The developed high-volume bioaerosol sampler will be useful in detecting viruses through PCR analysis because it can collect bioaerosols within a specific size range.
Highlights We designed 3-stage high-volume bioaerosol sampler with size-selective sampling. The sampler draws air at a high flow rate of 1000 L/min. We simulated the suspension of bioparticles attached to other particles. We analyzed the bioaerosol samples collected in each stage via PCR method.
High-volume sampler for size-selective sampling of bioaerosols including viruses
https://orcid.org/0000-0002-8365-5760
https://orcid.org/0000-0002-7419-021X
https://orcid.org/0000-0001-8197-0401
https://orcid.org/0000-0002-6005-2991
https://orcid.org/0000-0003-1804-5373
Abstract Owing to the recent global spread of the new coronavirus SARS-CoV-2, the development of technology to effectively detect viruses in crowded public places is urgently needed. In this study, a three-stage high-volume bioaerosol sampler was developed for the size-selective sampling of bioaerosols through the suction of air at a high flow rate of 1000 L/min. In stage 1, an omnidirectional inlet cyclone separator that can draw air from all directions was applied to collect bioaerosols larger than 10 μm in the collection fluid. In stage 2, an axial flow cyclone separator was used to collect bioaerosols sized between 2.5 and 10 μm in the collection fluid. In stage 3, bioaerosols smaller than 2.5 μm were collected on a filter and extracted in a solution through an elution process using a sodium phosphate buffer. To simulate the suspension of bioparticles including viruses that are attached to other particles in the atmosphere, the aerosol samples were prepared by coagulating aerosolized bacteriophages with Arizona test dust. Then, the coagulated particles were collected for 30 min using the developed bioaerosol sampler, and the samples collected in each stage were analyzed via polymerase chain reaction (PCR) method. The PCR analysis results confirmed that the high-volume bioaerosol sampler enables size-selective bioaerosol sampling even at a high airflow rate of 1000 L/min. The developed high-volume bioaerosol sampler will be useful in detecting viruses through PCR analysis because it can collect bioaerosols within a specific size range.
Highlights We designed 3-stage high-volume bioaerosol sampler with size-selective sampling. The sampler draws air at a high flow rate of 1000 L/min. We simulated the suspension of bioparticles attached to other particles. We analyzed the bioaerosol samples collected in each stage via PCR method.
High-volume sampler for size-selective sampling of bioaerosols including viruses
Lim, Jun-Hyung (author) / Nam, Sang Hwan (author) / Kim, Jongwoo (author) / Kim, Nam Hoon (author) / Park, Gun-Soo (author) / Maeng, Jin-Soo (author) / Yook, Se-Jin (author)
Atmospheric Environment ; 265
2021-09-08
Article (Journal)
Electronic Resource
English
| Wiley | 2011