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Biomimetic robust self-healing of Bacillus Subtilis immobilized through sisal fiber for next-generation concrete infrastructure
Graphical abstract Display Omitted
Highlights Bacillus Subtilis based bio-healable concrete was analyzed against two successive damage cycles for the very first time. Sisal fiber proved as a potential immobilizer for Bacillus Subtilis to develop robust self-healing concrete. Average crack healing width of 0.48 mm and 0.28 mm was attained after the 1st and 2nd healing phases. SEM, EDS, XRD, TGA, FTIR, and Raman spectroscopy affirmed bio-mediated calcite precipitation.
Abstract Bio-healing is now believed as a promising technique for the repair of concrete cracks. However, the healing capability of bacterial mixes against repeated damage cycles is still questionable. This study aims to investigate the robustness of bio-healable concrete against two successive damage cycles using Bacillus Subtilis immobilized via sisal fiber. The formulated bio-concrete exhibited improved mechanical properties and attained a maximum of 13.96 % and 36.82 % enhancement in terms of compression strength and split tensile strength. Self-healing efficacy was assessed by quantifying the average crack healing width and the recovered strength in compression during the healing phase. Results revealed that sisal fiber efficiently preserves spores of the bacillus strain and enhances its bio-metabolic potential to precipitate calcite for sealing concrete cracks. The average crack of 0.48 mm width was successfully healed in the first damage cycle whereas the complete repair of a 0.28 mm wide crack on average was attained in the healing phase of the second damage cycle. The maximum recovered compressive strength was 82.65 % and 50.42 % in the two successive damage cycles through bio-healing. The crack-healing precipitate was identified as calcite crystals through forensic examination. Consequently, immobilizing Bacillus Subtilis with sisal fiber was proved as a viable approach for improving the mechanical properties of concrete and inducing robust self-healing in repeated damages.
Biomimetic robust self-healing of Bacillus Subtilis immobilized through sisal fiber for next-generation concrete infrastructure
Graphical abstract Display Omitted
Highlights Bacillus Subtilis based bio-healable concrete was analyzed against two successive damage cycles for the very first time. Sisal fiber proved as a potential immobilizer for Bacillus Subtilis to develop robust self-healing concrete. Average crack healing width of 0.48 mm and 0.28 mm was attained after the 1st and 2nd healing phases. SEM, EDS, XRD, TGA, FTIR, and Raman spectroscopy affirmed bio-mediated calcite precipitation.
Abstract Bio-healing is now believed as a promising technique for the repair of concrete cracks. However, the healing capability of bacterial mixes against repeated damage cycles is still questionable. This study aims to investigate the robustness of bio-healable concrete against two successive damage cycles using Bacillus Subtilis immobilized via sisal fiber. The formulated bio-concrete exhibited improved mechanical properties and attained a maximum of 13.96 % and 36.82 % enhancement in terms of compression strength and split tensile strength. Self-healing efficacy was assessed by quantifying the average crack healing width and the recovered strength in compression during the healing phase. Results revealed that sisal fiber efficiently preserves spores of the bacillus strain and enhances its bio-metabolic potential to precipitate calcite for sealing concrete cracks. The average crack of 0.48 mm width was successfully healed in the first damage cycle whereas the complete repair of a 0.28 mm wide crack on average was attained in the healing phase of the second damage cycle. The maximum recovered compressive strength was 82.65 % and 50.42 % in the two successive damage cycles through bio-healing. The crack-healing precipitate was identified as calcite crystals through forensic examination. Consequently, immobilizing Bacillus Subtilis with sisal fiber was proved as a viable approach for improving the mechanical properties of concrete and inducing robust self-healing in repeated damages.
Biomimetic robust self-healing of Bacillus Subtilis immobilized through sisal fiber for next-generation concrete infrastructure
Amjad, Hassan (author) / Arsalan Khushnood, Rao (author) / Ali Memon, Shazim (author)
2023-01-02
Article (Journal)
Electronic Resource
English
OPC , Ordinary Portland Cement , ASTM , American Society for Testing and Materials , SF , Sisal Fiber , PP , Polypropylene , PVA , Polyvinyl Alcohol , TSB , Tryptone Soya Broth , BS , Bacillus Subtilis , OD , Optical Density , BISF , Bacillus Subtilis Immobilized in Sisal Fiber , TGA , Thermal Gravimetric Analysis , FTIR , Fourier Transformed Infrared , SEM , Scanning Electron Microscope , EDS , Energy Dispersive Spectroscopy , XRD , X-ray Diffraction , RCS , Recovered Compressive Strength , UPV , Ultrasonic Pulse Velocity , HE , Healing Extent , PCM , Plain Control Mix , FCM , Fiber Control Mix , PBM , Plain Bacterial Mix , FBM , Fiber Bacterial Mix , Self-healing concrete , Sisal fiber , Autonomous healing , Rigor healing , Multiple damages
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