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Optimum immobilization of Candida rugosa lipase on Celite by RSM
AbstractImmobilization of Candida rugosa lipase (lipase AY-30) by adsorption on Celite led to a markedly improved performance of the enzyme. Immobilization conditions and characterization of the immobilized enzyme were investigated. Lipase activity was measured with glycerol tributyrate as substrate. Response surface methodology (RSM) and 3-level–3-factor fractional factorial design were employed to evaluate the effects of immobilization parameters, such as immobilization time (30–90 min), immobilization temperature (0–20 °C), and enzyme/support ratio (0.3–0.5, w/w), on the specific activity of immobilized lipase. Based on the analysis of ridge max, the optimum immobilization conditions were as follows: immobilization time 59.1 min, immobilization temperature 10.7 °C, and enzyme/support ratio 0.5 (w/w); the highest specific activity obtained was 18.16 U/mg-protein with activity yield of 34.1%.
Optimum immobilization of Candida rugosa lipase on Celite by RSM
AbstractImmobilization of Candida rugosa lipase (lipase AY-30) by adsorption on Celite led to a markedly improved performance of the enzyme. Immobilization conditions and characterization of the immobilized enzyme were investigated. Lipase activity was measured with glycerol tributyrate as substrate. Response surface methodology (RSM) and 3-level–3-factor fractional factorial design were employed to evaluate the effects of immobilization parameters, such as immobilization time (30–90 min), immobilization temperature (0–20 °C), and enzyme/support ratio (0.3–0.5, w/w), on the specific activity of immobilized lipase. Based on the analysis of ridge max, the optimum immobilization conditions were as follows: immobilization time 59.1 min, immobilization temperature 10.7 °C, and enzyme/support ratio 0.5 (w/w); the highest specific activity obtained was 18.16 U/mg-protein with activity yield of 34.1%.
Optimum immobilization of Candida rugosa lipase on Celite by RSM
Chang, Shuo-Fen (author) / Chang, Shu-Wei (author) / Yen, Yue-Horng (author) / Shieh, Chwen-Jen (author)
Applied Clay Science ; 37 ; 67-73
2006-12-02
7 pages
Article (Journal)
Electronic Resource
English
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