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Silylation of mica for lipase immobilization as biocatalysts in esterification
AbstractMica was modified either by acid treatment, grafting with aminopropyl-, octyl-, vinyl-, mercapto- and glycidoxy-triethoxysilanes, and activation of pre-treated support with glutaraldehyde (Glu). The derivatives were characterized by X-ray diffraction (XRD), infra-red spectroscopy (FTIR), surface area and porosity analysis, scanning electron microscopy coupled with energy dispersive X-ray (SEM-EDX) and transmission electron microscopy (TEM) techniques. The modified micas were used for immobilization of lipase from Candida rugosa (CRL). Activity of the lipase was determined by esterification and exhibited the improved activity than the free enzyme following the order; Amino-CRL>Glu-Amino-CRL>Octyl-CRL>Vinyl-CRL>Glycidoxy-CRL>Mercapto-CRL>Mica-CRL. Lipase immobilized mica showed enhanced protein loading (up to 8.22mg protein/g support) and immobilization (up to 78%) compared to the free lipase and unmodified mica.
Silylation of mica for lipase immobilization as biocatalysts in esterification
AbstractMica was modified either by acid treatment, grafting with aminopropyl-, octyl-, vinyl-, mercapto- and glycidoxy-triethoxysilanes, and activation of pre-treated support with glutaraldehyde (Glu). The derivatives were characterized by X-ray diffraction (XRD), infra-red spectroscopy (FTIR), surface area and porosity analysis, scanning electron microscopy coupled with energy dispersive X-ray (SEM-EDX) and transmission electron microscopy (TEM) techniques. The modified micas were used for immobilization of lipase from Candida rugosa (CRL). Activity of the lipase was determined by esterification and exhibited the improved activity than the free enzyme following the order; Amino-CRL>Glu-Amino-CRL>Octyl-CRL>Vinyl-CRL>Glycidoxy-CRL>Mercapto-CRL>Mica-CRL. Lipase immobilized mica showed enhanced protein loading (up to 8.22mg protein/g support) and immobilization (up to 78%) compared to the free lipase and unmodified mica.
Silylation of mica for lipase immobilization as biocatalysts in esterification
Zaidan, Uswatun Hasanah (author) / Rahman, Mohd Basyaruddin Abdul (author) / Basri, Mahiran (author) / Othman, Siti Salhah (author) / Rahman, Raja Noor Zaliha Raja Abdul (author) / Salleh, Abu Bakar (author)
Applied Clay Science ; 47 ; 276-282
2009-11-04
7 pages
Article (Journal)
Electronic Resource
English
Silylation of mica for lipase immobilization as biocatalysts in esterification
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