A platform for research: civil engineering, architecture and urbanism
A platform for research: civil engineering, architecture and urbanism
Cometabolism of 17α-ethynylestradiol by nitrifying bacteria depends on reducing power availability and leads to elevated nitric oxide formation
https://orcid.org/0000-0001-9811-3828
Graphical abstract Display Omitted
Highlights NO increased during 17α-ethynylestradiol (EE2) cometabolism by N. europaea. nirK cluster genes were up-regulated during EE2 cometabolism by N. europaea. EE2 cometabolism depends on reducing power supplied by ammonia oxidation. EE2 cometabolic kinetics were estimated considering reductant availability. Nine transformation products of EE2 were identified including five newly reported.
Abstract 17α-ethynylestradiol (EE2) is a priority emerging contaminant (EC) in diverse environments that can be cometabolized by ammonia oxidizing bacteria (AOB). However, its transformation kinetics and the underlying molecular mechanism are unclear. In this study, kinetic parameters, including maximum specific EE2 transformation rate, EE2 half-saturation coefficient, and EE2transformation capacity of AOBwere obtained by using the model AOB strain, Nitrosomonas europaea 19718. The relationship between EE2 cometabolism and ammonia oxidation was divided into three phases according to reducing power availability, namely “activation”, “coupling”, and “saturation”. Specifically, there was a universal lag of EE2 transformation after ammonia oxidation was initiated, suggesting that sufficient reducing power (approximately 0.95 ± 0.06 mol NADH/L) was required to activate EE2 cometabolism. Interestingly, nitric oxide emission increased by 12 ± 2% during EE2 cometabolism, along with significantly upregulated nirK cluster genes. The findings are of importance to understanding the cometabolic behavior and mechanism of EE2 in natural and engineered environments. Maintaining relatively high and stable reducing power supply from ammonia oxidation can potentially improve the cometabolic removal of EE2 and other ECs during wastewater nitrification processes.
Cometabolism of 17α-ethynylestradiol by nitrifying bacteria depends on reducing power availability and leads to elevated nitric oxide formation
https://orcid.org/0000-0001-9811-3828
Graphical abstract Display Omitted
Highlights NO increased during 17α-ethynylestradiol (EE2) cometabolism by N. europaea. nirK cluster genes were up-regulated during EE2 cometabolism by N. europaea. EE2 cometabolism depends on reducing power supplied by ammonia oxidation. EE2 cometabolic kinetics were estimated considering reductant availability. Nine transformation products of EE2 were identified including five newly reported.
Abstract 17α-ethynylestradiol (EE2) is a priority emerging contaminant (EC) in diverse environments that can be cometabolized by ammonia oxidizing bacteria (AOB). However, its transformation kinetics and the underlying molecular mechanism are unclear. In this study, kinetic parameters, including maximum specific EE2 transformation rate, EE2 half-saturation coefficient, and EE2transformation capacity of AOBwere obtained by using the model AOB strain, Nitrosomonas europaea 19718. The relationship between EE2 cometabolism and ammonia oxidation was divided into three phases according to reducing power availability, namely “activation”, “coupling”, and “saturation”. Specifically, there was a universal lag of EE2 transformation after ammonia oxidation was initiated, suggesting that sufficient reducing power (approximately 0.95 ± 0.06 mol NADH/L) was required to activate EE2 cometabolism. Interestingly, nitric oxide emission increased by 12 ± 2% during EE2 cometabolism, along with significantly upregulated nirK cluster genes. The findings are of importance to understanding the cometabolic behavior and mechanism of EE2 in natural and engineered environments. Maintaining relatively high and stable reducing power supply from ammonia oxidation can potentially improve the cometabolic removal of EE2 and other ECs during wastewater nitrification processes.
Cometabolism of 17α-ethynylestradiol by nitrifying bacteria depends on reducing power availability and leads to elevated nitric oxide formation
https://orcid.org/0000-0001-9811-3828
Graphical abstract Display Omitted
Highlights NO increased during 17α-ethynylestradiol (EE2) cometabolism by N. europaea. nirK cluster genes were up-regulated during EE2 cometabolism by N. europaea. EE2 cometabolism depends on reducing power supplied by ammonia oxidation. EE2 cometabolic kinetics were estimated considering reductant availability. Nine transformation products of EE2 were identified including five newly reported.
Abstract 17α-ethynylestradiol (EE2) is a priority emerging contaminant (EC) in diverse environments that can be cometabolized by ammonia oxidizing bacteria (AOB). However, its transformation kinetics and the underlying molecular mechanism are unclear. In this study, kinetic parameters, including maximum specific EE2 transformation rate, EE2 half-saturation coefficient, and EE2transformation capacity of AOBwere obtained by using the model AOB strain, Nitrosomonas europaea 19718. The relationship between EE2 cometabolism and ammonia oxidation was divided into three phases according to reducing power availability, namely “activation”, “coupling”, and “saturation”. Specifically, there was a universal lag of EE2 transformation after ammonia oxidation was initiated, suggesting that sufficient reducing power (approximately 0.95 ± 0.06 mol NADH/L) was required to activate EE2 cometabolism. Interestingly, nitric oxide emission increased by 12 ± 2% during EE2 cometabolism, along with significantly upregulated nirK cluster genes. The findings are of importance to understanding the cometabolic behavior and mechanism of EE2 in natural and engineered environments. Maintaining relatively high and stable reducing power supply from ammonia oxidation can potentially improve the cometabolic removal of EE2 and other ECs during wastewater nitrification processes.
Cometabolism of 17α-ethynylestradiol by nitrifying bacteria depends on reducing power availability and leads to elevated nitric oxide formation
Sheng, Qi (author) / Yi, Ming (author) / Men, Yujie (author) / Lu, Huijie (author)
2021-03-13
Article (Journal)
Electronic Resource
English
Chlorinated solvents cometabolism by an enriched nitrifying bacterial consortium
| British Library Conference Proceedings | 2001