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Non-covalent binding interaction between phthalic acid esters and DNA
Graphical abstract Display Omitted
Highlights ·A primary investigation into phthalic acid esters-DNA interaction mechanism. ·Phthalic acid esters preferentially bind to the thymine-rich region of DNA in the minor groove. ·Phthalic acid esters-DNA binding strength is negatively correlated with electrostatic surface potential. ·Hydrogen bond and van der Waals force are the key binding forces.
Abstract The interaction of phthalic acid esters (PAEs) with DNA is known to be responsible for the disruption of endocrine functions and the teratogenic and carcinogenic effects. However, the binding strength and mechanism of this important process has often been neglected. Here, we confirmed the binding interaction between PAEs and DNA via fluorescence titration quenching experiment. The linear fitting curve proved that PAEs could bind to DNA, and the binding constants (K A) were 4.11 × 105, 1.04 × 105, 7.60 × 104, 1.99 × 104, and 1.42 × 103 L/mol for diethyl phthalate (DEP), bis(2-ethylhexyl) phthalate (DEHP), dibutyl phthalate (DBP), dimethyl phthalate (DMP), and benzyl butyl phthalate (BBP), respectively. DNA melting point, UV–vis spectra and Fourier transform infrared spectroscopy (FTIR) analyses revealed that PAEs interact predominantly with thymines in the DNA minor groove. Quantum chemical calculations showed that hydrogen bonding and van der Waals force formation between PAEs and DNA bases dominated the binding interaction. However, PAEs-DNA binding did not induce any DNA conformation change since the circular dichroism and FTIR spectra of B-DNA were not change. The electrostatic surface potential (ESP) might act an important role in PAEs-DNA binding interaction. This work will broaden our understanding of the interaction between PAEs and genetic materials.
Non-covalent binding interaction between phthalic acid esters and DNA
Graphical abstract Display Omitted
Highlights ·A primary investigation into phthalic acid esters-DNA interaction mechanism. ·Phthalic acid esters preferentially bind to the thymine-rich region of DNA in the minor groove. ·Phthalic acid esters-DNA binding strength is negatively correlated with electrostatic surface potential. ·Hydrogen bond and van der Waals force are the key binding forces.
Abstract The interaction of phthalic acid esters (PAEs) with DNA is known to be responsible for the disruption of endocrine functions and the teratogenic and carcinogenic effects. However, the binding strength and mechanism of this important process has often been neglected. Here, we confirmed the binding interaction between PAEs and DNA via fluorescence titration quenching experiment. The linear fitting curve proved that PAEs could bind to DNA, and the binding constants (K A) were 4.11 × 105, 1.04 × 105, 7.60 × 104, 1.99 × 104, and 1.42 × 103 L/mol for diethyl phthalate (DEP), bis(2-ethylhexyl) phthalate (DEHP), dibutyl phthalate (DBP), dimethyl phthalate (DMP), and benzyl butyl phthalate (BBP), respectively. DNA melting point, UV–vis spectra and Fourier transform infrared spectroscopy (FTIR) analyses revealed that PAEs interact predominantly with thymines in the DNA minor groove. Quantum chemical calculations showed that hydrogen bonding and van der Waals force formation between PAEs and DNA bases dominated the binding interaction. However, PAEs-DNA binding did not induce any DNA conformation change since the circular dichroism and FTIR spectra of B-DNA were not change. The electrostatic surface potential (ESP) might act an important role in PAEs-DNA binding interaction. This work will broaden our understanding of the interaction between PAEs and genetic materials.
Non-covalent binding interaction between phthalic acid esters and DNA
Cheng, Hao (author) / Qin, Chao (author) / Yang, Bing (author) / Hu, Xiaojie (author) / Gatheru Waigi, Michael (author) / Vasilyeva, Galina K. (author) / Gao, Yanzheng (author)
2022-01-12
Article (Journal)
Electronic Resource
English
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