A platform for research: civil engineering, architecture and urbanism
A platform for research: civil engineering, architecture and urbanism
Managing two simultaneous issues in concrete repair: Healing microcracks and controlling pathogens
https://orcid.org/0009-0008-8657-5273
https://orcid.org/0000-0002-7402-1280
https://orcid.org/0000-0002-3166-1528
Abstract Maintaining concrete structures by using bacterial agents to repair microcracks is a promising strategy for maximizing their lifespan. A non-ureolytic and alkali-tolerant B6 strain, newly isolated from paddy soil, was tested for microbiologically induced calcium carbonate precipitation (MICP) performance along with its antibacterial activity for pathogen removals. Whole genome and bioinformatic analyses showed that our B6 strain could be designated as Bacillus altitudinis with its 16S rDNA and average nucleotide identity. Field emission scanning electron microscopy (FE-SEM) and X-ray diffractometry (XRD) revealed that the B6 strain could form vaterite and produce extracellular polymeric substances, thereby contributing to excellent biofilm formation, even under high pH conditions. The MICP in the B6 cells, inoculated on cracked mortars, could repair microcracks (0.3 mm) within 14 days. The presence of vaterite and survivability by the B6 cells inside the healed area was verified using energy-dispersive X-ray spectroscopy and FE-SEM. Antibacterial activity was determined using the supernatant of the B6 cells grown in rich media at pH 8 and showed that the cell-free extract could kill Gram-positive bacteria, including Staphylococcus aureus and Enterococcus faecalis, by damaging their cellular membranes. Thermostable thiocillin, a putatively secreted antibacterial compound, was identified using biosynthetic gene cluster analyses for secondary metabolites and chemical analysis using reversed-phase high-performance liquid chromatography (RP-HPLC/UV) and ultra-performance liquid chromatography (UPLC)–mass spectrometry (MS). Our data demonstrated that the MICP and antibacterial activities of the B6 strain could be promising components for repairing microcracks and controlling the pathogen contamination of concrete.
Graphical abstract Display Omitted
Highlights The calcium carbonate precipitation (CCP)-capability of Bacillus altitudinins B6 has shown promise in repairing microcracks. The bactericidal substance of the B6 strain exhibits superior antibacterial activity against Gram-positive bacteria. Thermostable thiocillin-like peptide from the B6 strain was identified through chemical analyses. The CCP-capability and antibacterial activity of the B6 strain could expand the cementitious structure's maintenance lifespan.
Managing two simultaneous issues in concrete repair: Healing microcracks and controlling pathogens
https://orcid.org/0009-0008-8657-5273
https://orcid.org/0000-0002-7402-1280
https://orcid.org/0000-0002-3166-1528
Abstract Maintaining concrete structures by using bacterial agents to repair microcracks is a promising strategy for maximizing their lifespan. A non-ureolytic and alkali-tolerant B6 strain, newly isolated from paddy soil, was tested for microbiologically induced calcium carbonate precipitation (MICP) performance along with its antibacterial activity for pathogen removals. Whole genome and bioinformatic analyses showed that our B6 strain could be designated as Bacillus altitudinis with its 16S rDNA and average nucleotide identity. Field emission scanning electron microscopy (FE-SEM) and X-ray diffractometry (XRD) revealed that the B6 strain could form vaterite and produce extracellular polymeric substances, thereby contributing to excellent biofilm formation, even under high pH conditions. The MICP in the B6 cells, inoculated on cracked mortars, could repair microcracks (0.3 mm) within 14 days. The presence of vaterite and survivability by the B6 cells inside the healed area was verified using energy-dispersive X-ray spectroscopy and FE-SEM. Antibacterial activity was determined using the supernatant of the B6 cells grown in rich media at pH 8 and showed that the cell-free extract could kill Gram-positive bacteria, including Staphylococcus aureus and Enterococcus faecalis, by damaging their cellular membranes. Thermostable thiocillin, a putatively secreted antibacterial compound, was identified using biosynthetic gene cluster analyses for secondary metabolites and chemical analysis using reversed-phase high-performance liquid chromatography (RP-HPLC/UV) and ultra-performance liquid chromatography (UPLC)–mass spectrometry (MS). Our data demonstrated that the MICP and antibacterial activities of the B6 strain could be promising components for repairing microcracks and controlling the pathogen contamination of concrete.
Graphical abstract Display Omitted
Highlights The calcium carbonate precipitation (CCP)-capability of Bacillus altitudinins B6 has shown promise in repairing microcracks. The bactericidal substance of the B6 strain exhibits superior antibacterial activity against Gram-positive bacteria. Thermostable thiocillin-like peptide from the B6 strain was identified through chemical analyses. The CCP-capability and antibacterial activity of the B6 strain could expand the cementitious structure's maintenance lifespan.
Managing two simultaneous issues in concrete repair: Healing microcracks and controlling pathogens
https://orcid.org/0009-0008-8657-5273
https://orcid.org/0000-0002-7402-1280
https://orcid.org/0000-0002-3166-1528
Abstract Maintaining concrete structures by using bacterial agents to repair microcracks is a promising strategy for maximizing their lifespan. A non-ureolytic and alkali-tolerant B6 strain, newly isolated from paddy soil, was tested for microbiologically induced calcium carbonate precipitation (MICP) performance along with its antibacterial activity for pathogen removals. Whole genome and bioinformatic analyses showed that our B6 strain could be designated as Bacillus altitudinis with its 16S rDNA and average nucleotide identity. Field emission scanning electron microscopy (FE-SEM) and X-ray diffractometry (XRD) revealed that the B6 strain could form vaterite and produce extracellular polymeric substances, thereby contributing to excellent biofilm formation, even under high pH conditions. The MICP in the B6 cells, inoculated on cracked mortars, could repair microcracks (0.3 mm) within 14 days. The presence of vaterite and survivability by the B6 cells inside the healed area was verified using energy-dispersive X-ray spectroscopy and FE-SEM. Antibacterial activity was determined using the supernatant of the B6 cells grown in rich media at pH 8 and showed that the cell-free extract could kill Gram-positive bacteria, including Staphylococcus aureus and Enterococcus faecalis, by damaging their cellular membranes. Thermostable thiocillin, a putatively secreted antibacterial compound, was identified using biosynthetic gene cluster analyses for secondary metabolites and chemical analysis using reversed-phase high-performance liquid chromatography (RP-HPLC/UV) and ultra-performance liquid chromatography (UPLC)–mass spectrometry (MS). Our data demonstrated that the MICP and antibacterial activities of the B6 strain could be promising components for repairing microcracks and controlling the pathogen contamination of concrete.
Graphical abstract Display Omitted
Highlights The calcium carbonate precipitation (CCP)-capability of Bacillus altitudinins B6 has shown promise in repairing microcracks. The bactericidal substance of the B6 strain exhibits superior antibacterial activity against Gram-positive bacteria. Thermostable thiocillin-like peptide from the B6 strain was identified through chemical analyses. The CCP-capability and antibacterial activity of the B6 strain could expand the cementitious structure's maintenance lifespan.
Managing two simultaneous issues in concrete repair: Healing microcracks and controlling pathogens
Min, Jihyeon (author) / Son, Yongjun (author) / Jang, Indong (author) / Yi, Chongku (author) / Park, Woojun (author)
2024-01-19
Article (Journal)
Electronic Resource
English
Diffusion mechanism of healing microcracks in wearing
| British Library Online Contents | 1997
| British Library Online Contents | 2017