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Light-driven nitrous oxide production via autotrophic denitrification by self-photosensitized Thiobacillus denitrificans
Abstract N2O (Nitrous oxide, a booster oxidant in rockets) has attracted increasing interest as a means of enhancing energy production, and it can be produced by nitrate (NO3 −) reduction in NO3 −-loading wastewater. However, conventional denitrification processes are often limited by the lack of bioavailable electron donors. In this study, we innovatively propose a self-photosensitized nonphototrophic Thiobacillus denitrificans (T. denitrificans-CdS) that is capable of NO3 − reduction and N2O production driven by light. The system converted >72.1 ± 1.1% of the NO3 −-N input to N2ON, and the ratio of N2O-N in gaseous products was >96.4 ± 0.4%. The relative transcript abundance of the genes encoding the denitrifying proteins in T. denitrificans-CdS after irradiation was significantly upregulated. The photoexcited electrons acted as the dominant electron sources for NO3 − reduction by T. denitrificans-CdS. This study provides the first proof of concept for sustainable and low-cost autotrophic denitrification to generate N2O driven by light. The findings also have strong implications for sustainable environmental management because the sunlight-triggered denitrification reaction driven by nonphototrophic microorganisms may widely occur in nature, particularly in a semiconductive mineral-enriched aqueous environment.
Graphical abstract Display Omitted
Highlights It is the first proof of concept evidence for nonphototrophic denitrification driven by light. The system converts NO3 − to N2O with a purity >96%. A high quantum efficiency of 2.0% in light-driven nitrate reduction is achieved. Photoexcited electrons are the dominant electron source for denitrification in this system.
Light-driven nitrous oxide production via autotrophic denitrification by self-photosensitized Thiobacillus denitrificans
Abstract N2O (Nitrous oxide, a booster oxidant in rockets) has attracted increasing interest as a means of enhancing energy production, and it can be produced by nitrate (NO3 −) reduction in NO3 −-loading wastewater. However, conventional denitrification processes are often limited by the lack of bioavailable electron donors. In this study, we innovatively propose a self-photosensitized nonphototrophic Thiobacillus denitrificans (T. denitrificans-CdS) that is capable of NO3 − reduction and N2O production driven by light. The system converted >72.1 ± 1.1% of the NO3 −-N input to N2ON, and the ratio of N2O-N in gaseous products was >96.4 ± 0.4%. The relative transcript abundance of the genes encoding the denitrifying proteins in T. denitrificans-CdS after irradiation was significantly upregulated. The photoexcited electrons acted as the dominant electron sources for NO3 − reduction by T. denitrificans-CdS. This study provides the first proof of concept for sustainable and low-cost autotrophic denitrification to generate N2O driven by light. The findings also have strong implications for sustainable environmental management because the sunlight-triggered denitrification reaction driven by nonphototrophic microorganisms may widely occur in nature, particularly in a semiconductive mineral-enriched aqueous environment.
Graphical abstract Display Omitted
Highlights It is the first proof of concept evidence for nonphototrophic denitrification driven by light. The system converts NO3 − to N2O with a purity >96%. A high quantum efficiency of 2.0% in light-driven nitrate reduction is achieved. Photoexcited electrons are the dominant electron source for denitrification in this system.
Light-driven nitrous oxide production via autotrophic denitrification by self-photosensitized Thiobacillus denitrificans
Chen, Man (author) / Zhou, Xiao-Fang (author) / Yu, Yu-Qing (author) / Liu, Xing (author) / Zeng, Raymond Jian-Xiong (author) / Zhou, Shun-Gui (author) / He, Zhen (author)
Environmental International ; 127 ; 353-360
2019-03-19
8 pages
Article (Journal)
Electronic Resource
English
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