Preparation of cetylpyridinium montmorillonite for antibacterial applications: Fid-Bau Portal

Preparation of cetylpyridinium montmorillonite for antibacterial applications

Özdemir, Günseli / Yapar, Saadet / Limoncu, Mine Hoşgör

Abstract The antibacterial activities of cetylpyridinium-montmorillonites (CP⁺-Mt) were tested on Staphylococcus aureus and Pseudomonas aeruginosa. The Mt were prepared by using the five different CP⁺ amounts of 0.5, 0.7, 1.0, 1.5, and 2.0 times of cation exchange capacities (CEC) of Na⁺-Mt. Desorption of CP⁺ from the surface was also determined by successive adsorption–desorption experiments. The antibacterial activity tests were conducted by using Na⁺-Mt and CP⁺-Mt through the disk diffusion (Kirby–Bauer) method against the P. aeruginosa ATCC 27853, and S. aureus ATCC 29213 strains. XRD analyses of the CP⁺-Mt showed that basal spacing regularly increased by increasing the amount of CP⁺ cations. Adsorption/desorption studies revealed that desorption occurred only in 2.0CEC CP⁺-Mt by dilution with water and in 1.0CEC CP⁺-Mt at a pH of 2.0. Na⁺-Mt exhibited no antibacterial activity against both bacteria. All of the CP⁺-Mt samples prepared were active against S. aureus, whereas they had no antibacterial activity against P. aeruginosa. Minimum inhibitory concentration (MIC) was found to be 1mg/plate against S. aureus, determined with 0.5CEC CP⁺-Mt. Because nearly no desorption of CP⁺ was observed, the antibacterial activity was attributed to the CP⁺ bound to the Mt surface.

Highlights ► Preparation of cetylpyridinium montmorillonite (CP⁺-Mt) ► Antibacterial tests of CP⁺-Mt against S. aureus and P. aeruginosa ► 0.5CEC to 2.0CEC of CP⁺-Mt prepared is effective against S. aureus. ► CP⁺-Mt has the potential to be used in long lasting antibacterial applications.