A platform for research: civil engineering, architecture and urbanism
A platform for research: civil engineering, architecture and urbanism
Vertical distribution of airborne microorganisms over forest environments: A potential source of ice-nucleating bioaerosols
https://orcid.org/0000-0001-6306-0746
https://orcid.org/0000-0003-0210-2628
Abstract Airborne microorganisms transported from forested areas can influence cloud formation by forming ice nuclei. However, the vertical transportation of airborne microorganisms over forested areas is not well understood. We collected aerosols at three heights, [ground (2 m), canopy top (20 m), and above canopy (500 m)] during the summer, fall and winter, to analyze the airborne microbial communities that were distributed vertically over the forest. During summer and fall, the microbial particles maintained similar concentrations in the forest zone (canopy top and ground) and decreased to 1/10th of the microbial concentration in the above canopy area. The particle concentrations in winter indicated efficient vertical mixing below 500 m. High-throughput DNA sequencing revealed that the airborne microbial communities were composed of terrestrial and phyllospheric species associated with the degradation of decaying plant litters. Regardless of the three seasons, the above canopy was dominated by atmospheric stress-resistant bacteria from the phyla Actinobacteria and Firmicutes. Unlike bacteria, the mushroom-type fungal members of Agaricomycetes grew in relative abundance above the canopy, primarily throughout the summer and winter, while mold-type fungal Dothideomycetes species were often found at all three heights during the fall. The Fusarium, Pseudomonas, and Bacillus isolates, which were obtained from air samples at three heights, indicated high activities of ice nucleation in the water-drop freezing assay. Therefore, ice-nucleating microbial taxa likely originated from fungal and bacterial communities in the soil litter and plant surfaces of the phyllospheric environments.
Graphical abstract Display Omitted
Highlights Bioaerosols were collected from ground to high altitude at the forest environments. Terrestrial and phyllospheric bacteria were dominated vertically over forest. Mushroom- and mold-type fungi in airborne fungal communities changed seasonally. Ice-nucleating microorganisms were obtained from the aerosols at heights of 500 m.
Vertical distribution of airborne microorganisms over forest environments: A potential source of ice-nucleating bioaerosols
https://orcid.org/0000-0001-6306-0746
https://orcid.org/0000-0003-0210-2628
Abstract Airborne microorganisms transported from forested areas can influence cloud formation by forming ice nuclei. However, the vertical transportation of airborne microorganisms over forested areas is not well understood. We collected aerosols at three heights, [ground (2 m), canopy top (20 m), and above canopy (500 m)] during the summer, fall and winter, to analyze the airborne microbial communities that were distributed vertically over the forest. During summer and fall, the microbial particles maintained similar concentrations in the forest zone (canopy top and ground) and decreased to 1/10th of the microbial concentration in the above canopy area. The particle concentrations in winter indicated efficient vertical mixing below 500 m. High-throughput DNA sequencing revealed that the airborne microbial communities were composed of terrestrial and phyllospheric species associated with the degradation of decaying plant litters. Regardless of the three seasons, the above canopy was dominated by atmospheric stress-resistant bacteria from the phyla Actinobacteria and Firmicutes. Unlike bacteria, the mushroom-type fungal members of Agaricomycetes grew in relative abundance above the canopy, primarily throughout the summer and winter, while mold-type fungal Dothideomycetes species were often found at all three heights during the fall. The Fusarium, Pseudomonas, and Bacillus isolates, which were obtained from air samples at three heights, indicated high activities of ice nucleation in the water-drop freezing assay. Therefore, ice-nucleating microbial taxa likely originated from fungal and bacterial communities in the soil litter and plant surfaces of the phyllospheric environments.
Graphical abstract Display Omitted
Highlights Bioaerosols were collected from ground to high altitude at the forest environments. Terrestrial and phyllospheric bacteria were dominated vertically over forest. Mushroom- and mold-type fungi in airborne fungal communities changed seasonally. Ice-nucleating microorganisms were obtained from the aerosols at heights of 500 m.
Vertical distribution of airborne microorganisms over forest environments: A potential source of ice-nucleating bioaerosols
https://orcid.org/0000-0001-6306-0746
https://orcid.org/0000-0003-0210-2628
Abstract Airborne microorganisms transported from forested areas can influence cloud formation by forming ice nuclei. However, the vertical transportation of airborne microorganisms over forested areas is not well understood. We collected aerosols at three heights, [ground (2 m), canopy top (20 m), and above canopy (500 m)] during the summer, fall and winter, to analyze the airborne microbial communities that were distributed vertically over the forest. During summer and fall, the microbial particles maintained similar concentrations in the forest zone (canopy top and ground) and decreased to 1/10th of the microbial concentration in the above canopy area. The particle concentrations in winter indicated efficient vertical mixing below 500 m. High-throughput DNA sequencing revealed that the airborne microbial communities were composed of terrestrial and phyllospheric species associated with the degradation of decaying plant litters. Regardless of the three seasons, the above canopy was dominated by atmospheric stress-resistant bacteria from the phyla Actinobacteria and Firmicutes. Unlike bacteria, the mushroom-type fungal members of Agaricomycetes grew in relative abundance above the canopy, primarily throughout the summer and winter, while mold-type fungal Dothideomycetes species were often found at all three heights during the fall. The Fusarium, Pseudomonas, and Bacillus isolates, which were obtained from air samples at three heights, indicated high activities of ice nucleation in the water-drop freezing assay. Therefore, ice-nucleating microbial taxa likely originated from fungal and bacterial communities in the soil litter and plant surfaces of the phyllospheric environments.
Graphical abstract Display Omitted
Highlights Bioaerosols were collected from ground to high altitude at the forest environments. Terrestrial and phyllospheric bacteria were dominated vertically over forest. Mushroom- and mold-type fungi in airborne fungal communities changed seasonally. Ice-nucleating microorganisms were obtained from the aerosols at heights of 500 m.
Vertical distribution of airborne microorganisms over forest environments: A potential source of ice-nucleating bioaerosols
Maki, Teruya (author) / Hosaka, Kentaro (author) / Lee, Kevin C. (author) / Kawabata, Yasuhiro (author) / Kajino, Mizuo (author) / Uto, Maoto (author) / Kita, Kazuyuki (author) / Igarashi, Yasuhito (author)
Atmospheric Environment ; 302
2023-03-15
Article (Journal)
Electronic Resource
English
Contribution of bioaerosols to airborne particulate matter
| Taylor & Francis Verlag | 2020
Bioaerosols in Health-Care Environments
| British Library Online Contents | 2014
Bioaerosols in industrial environments: a review
| British Library Conference Proceedings | 2009
Bioaerosols in industrial environments: a review
| British Library Online Contents | 2009