A platform for research: civil engineering, architecture and urbanism
A platform for research: civil engineering, architecture and urbanism
DNA hypomethylation and its mediation in the effects of fine particulate air pollution on cardiovascular biomarkers: A randomized crossover trial
Abstract Background Short-term exposure to fine particulate matter (PM2.5) air pollution has been associated with altered DNA methylation in observational studies, but it remains unclear whether this change mediates the effects on cardiovascular biomarkers. Objective To examine the impact of ambient PM2.5 on gene-specific DNA methylation and its potential mediation in the acute effects of PM2.5 on cardiovascular biomarkers. Methods We designed a randomized, double-blind crossover trial using true or sham air purifiers for 48h among 35 healthy college students in Shanghai, China, in 2014. We measured blood global methylation estimated in long interspersed nucleotide element-1 (LINE‑1) and Alu repetitive elements, methylation in ten specific genes, and ten cardiovascular biomarkers. We used linear mixed-effect models to examine the associations between PM2.5 and methylation. We also performed causal mediation analyses to evaluate the potential mediation of methylation in the associations between PM2.5 and biomarkers. Results Air purification increased DNA methylation in repetitive elements and all candidate genes. An IQR increase (64μg/m3) in PM2.5 was significantly associated with reduction of methylation in LINE-1 (1.44%), one pro-inflammatory gene (CD40LG, 9.13%), two pro-coagulant genes (F3, 15.20%; SERPINE1, 3.69%), and two pro-vasoconstriction genes (ACE, 4.64%; EDN1, 9.74%). There was a significant mediated effect (17.82%, P =0.03) of PM2.5 on sCD40L protein through CD40LG hypomethylation. Hypomethylation in other candidate genes generally showed positive but non-significant mediation. Conclusions This intervention study provided robust human evidence that ambient PM2.5 could induce rapid decreases in DNA methylation and consequently partly mediate its effects on cardiovascular biomarkers.
Highlights It is unclear whether methylation mediates the cardiovascular effects of PM2.5. We tested methylation in 10 genes and biomarkers in a randomized controlled trial. PM2.5 may reduce DNA methylation in inflammation, coagulation and vasoconstriction. CD40LG hypomethylation may mediate the effect of PM2.5 on sCD40L protein.
DNA hypomethylation and its mediation in the effects of fine particulate air pollution on cardiovascular biomarkers: A randomized crossover trial
Abstract Background Short-term exposure to fine particulate matter (PM2.5) air pollution has been associated with altered DNA methylation in observational studies, but it remains unclear whether this change mediates the effects on cardiovascular biomarkers. Objective To examine the impact of ambient PM2.5 on gene-specific DNA methylation and its potential mediation in the acute effects of PM2.5 on cardiovascular biomarkers. Methods We designed a randomized, double-blind crossover trial using true or sham air purifiers for 48h among 35 healthy college students in Shanghai, China, in 2014. We measured blood global methylation estimated in long interspersed nucleotide element-1 (LINE‑1) and Alu repetitive elements, methylation in ten specific genes, and ten cardiovascular biomarkers. We used linear mixed-effect models to examine the associations between PM2.5 and methylation. We also performed causal mediation analyses to evaluate the potential mediation of methylation in the associations between PM2.5 and biomarkers. Results Air purification increased DNA methylation in repetitive elements and all candidate genes. An IQR increase (64μg/m3) in PM2.5 was significantly associated with reduction of methylation in LINE-1 (1.44%), one pro-inflammatory gene (CD40LG, 9.13%), two pro-coagulant genes (F3, 15.20%; SERPINE1, 3.69%), and two pro-vasoconstriction genes (ACE, 4.64%; EDN1, 9.74%). There was a significant mediated effect (17.82%, P =0.03) of PM2.5 on sCD40L protein through CD40LG hypomethylation. Hypomethylation in other candidate genes generally showed positive but non-significant mediation. Conclusions This intervention study provided robust human evidence that ambient PM2.5 could induce rapid decreases in DNA methylation and consequently partly mediate its effects on cardiovascular biomarkers.
Highlights It is unclear whether methylation mediates the cardiovascular effects of PM2.5. We tested methylation in 10 genes and biomarkers in a randomized controlled trial. PM2.5 may reduce DNA methylation in inflammation, coagulation and vasoconstriction. CD40LG hypomethylation may mediate the effect of PM2.5 on sCD40L protein.
DNA hypomethylation and its mediation in the effects of fine particulate air pollution on cardiovascular biomarkers: A randomized crossover trial
Chen, Renjie (author) / Meng, Xia (author) / Zhao, Ang (author) / Wang, Cuicui (author) / Yang, Changyuan (author) / Li, Huichu (author) / Cai, Jing (author) / Zhao, Zhuohui (author) / Kan, Haidong (author)
Environmental International ; 94 ; 614-619
2016-06-21
6 pages
Article (Journal)
Electronic Resource
English