A platform for research: civil engineering, architecture and urbanism
A platform for research: civil engineering, architecture and urbanism
Flexible and high-throughput sequencing of targeted genomic regions
The disclosure pertains to materials and methods for capturing a target genomic region, comprising hybridizing an extension probe and a ligation probe to target sequences that flank the target genomic region; elongating the 3' end of the extension probe until the 3' end of the elongated extension probe is adjacent to the 5' end of the ligation probe; and ligating the 3' end of the elongated extension probe with the 5' end of the ligation probe to produce a ligated probe. The ligated probe can be PCR amplified to produce copies of the target genomic region that can be detected or sequenced. Certain embodiments of the invention also provide methods of producing double stranded probes suitable for capturing and analyzing both strands of a target genomic region in a double stranded genomic DNA. The invention also provides kits for performing the methods disclosed herein.
Flexible and high-throughput sequencing of targeted genomic regions
The disclosure pertains to materials and methods for capturing a target genomic region, comprising hybridizing an extension probe and a ligation probe to target sequences that flank the target genomic region; elongating the 3' end of the extension probe until the 3' end of the elongated extension probe is adjacent to the 5' end of the ligation probe; and ligating the 3' end of the elongated extension probe with the 5' end of the ligation probe to produce a ligated probe. The ligated probe can be PCR amplified to produce copies of the target genomic region that can be detected or sequenced. Certain embodiments of the invention also provide methods of producing double stranded probes suitable for capturing and analyzing both strands of a target genomic region in a double stranded genomic DNA. The invention also provides kits for performing the methods disclosed herein.
Flexible and high-throughput sequencing of targeted genomic regions
NEVES LEANDRO GOMIDE (author) / PAYTON ADAM (author)
2022-01-20
Patent
Electronic Resource
English
IPC:
E04C
STRUCTURAL ELEMENTS
,
Bauelemente
FLEXIBLE AND HIGH-THROUGHPUT SEQUENCING OF TARGETED GENOMIC REGIONS
| European Patent Office | 2020
FLEXIBLE AND HIGH-THROUGHPUT SEQUENCING OF TARGETED GENOMIC REGIONS
| European Patent Office | 2023
FLEXIBLE AND HIGH-THROUGHPUT SEQUENCING OF TARGETED GENOMIC REGIONS
| European Patent Office | 2020
Flexible and high throughput sequencing of target genomic regions
| European Patent Office | 2022