A platform for research: civil engineering, architecture and urbanism
A platform for research: civil engineering, architecture and urbanism
Preparation method of multi-strain composite concrete self-repairing additive
The invention relates to the technical field of concrete additives, and particularly discloses a preparation method of a multi-strain composite concrete self-repairing additive, which comprises the following steps: preparing a casein tryptone soybean broth culture medium by using deionized water, and sterilizing at high temperature and high pressure; the method comprises the following steps: adding urea into a casein tryptone soybean broth culture medium subjected to high-temperature and high-pressure treatment to obtain a urea-casein tryptone soybean broth culture medium, and respectively culturing bacillus pasteurii and bacillus thuringiensis by using the urea-casein tryptone soybean broth culture medium, the method comprises the following steps: inoculating bacillus pasteurii and bacillus thuringiensis which are cultured for a certain period of time into a new urea-casein tryptone soybean broth culture medium according to a certain proportion, culturing for a certain period of time, adding a microbial carrier into the urea-casein tryptone soybean broth culture medium containing two bacteria, and soaking for a certain period of time; the soaked microbial carrier is taken out and dried, and the multi-strain composite concrete self-repairing additive is obtained. The additive is simple in preparation method and suitable for batch production.
本申请涉及混凝土添加剂技术领域，具体公开了一种多菌种复合混凝土自修复添加剂的制备方法，其包括使用去离子水配置胰酪胨大豆肉汤培养基并在高温高压下灭菌，在经过高温高压处理后的胰酪胨大豆肉汤培养基中加入尿素得到尿素‑胰酪胨大豆肉汤培养基，使用尿素‑胰酪胨大豆肉汤培养基分别培养巴氏芽孢杆菌与苏云金芽孢杆菌，将培养一定时间后的巴氏芽孢杆菌与苏云金芽孢杆菌按照一定比例接种到新的尿素‑胰酪胨大豆肉汤培养基后培养一定时间，随后将微生物载体加入含有两种细菌的尿素‑胰酪胨大豆肉汤培养基中浸泡一定时间，将浸泡后的微生物载体取出烘干即得到多菌种复合混凝土自修复添加剂，此添加剂制备方法简单，适宜于批量化生产。
Preparation method of multi-strain composite concrete self-repairing additive
The invention relates to the technical field of concrete additives, and particularly discloses a preparation method of a multi-strain composite concrete self-repairing additive, which comprises the following steps: preparing a casein tryptone soybean broth culture medium by using deionized water, and sterilizing at high temperature and high pressure; the method comprises the following steps: adding urea into a casein tryptone soybean broth culture medium subjected to high-temperature and high-pressure treatment to obtain a urea-casein tryptone soybean broth culture medium, and respectively culturing bacillus pasteurii and bacillus thuringiensis by using the urea-casein tryptone soybean broth culture medium, the method comprises the following steps: inoculating bacillus pasteurii and bacillus thuringiensis which are cultured for a certain period of time into a new urea-casein tryptone soybean broth culture medium according to a certain proportion, culturing for a certain period of time, adding a microbial carrier into the urea-casein tryptone soybean broth culture medium containing two bacteria, and soaking for a certain period of time; the soaked microbial carrier is taken out and dried, and the multi-strain composite concrete self-repairing additive is obtained. The additive is simple in preparation method and suitable for batch production.
本申请涉及混凝土添加剂技术领域，具体公开了一种多菌种复合混凝土自修复添加剂的制备方法，其包括使用去离子水配置胰酪胨大豆肉汤培养基并在高温高压下灭菌，在经过高温高压处理后的胰酪胨大豆肉汤培养基中加入尿素得到尿素‑胰酪胨大豆肉汤培养基，使用尿素‑胰酪胨大豆肉汤培养基分别培养巴氏芽孢杆菌与苏云金芽孢杆菌，将培养一定时间后的巴氏芽孢杆菌与苏云金芽孢杆菌按照一定比例接种到新的尿素‑胰酪胨大豆肉汤培养基后培养一定时间，随后将微生物载体加入含有两种细菌的尿素‑胰酪胨大豆肉汤培养基中浸泡一定时间，将浸泡后的微生物载体取出烘干即得到多菌种复合混凝土自修复添加剂，此添加剂制备方法简单，适宜于批量化生产。
Preparation method of multi-strain composite concrete self-repairing additive
一种多菌种复合混凝土自修复添加剂的制备方法
LU TAO (author) / GAO ZONGHONG (author) / CHEN JIE (author) / FANG SHICHANG (author) / TIAN YINGBING (author)
2023-03-10
Patent
Electronic Resource
Chinese
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