A platform for research: civil engineering, architecture and urbanism
A platform for research: civil engineering, architecture and urbanism
Preparation method of MICP combined waste molasses reinforced calcareous sand
The invention discloses a preparation method of MICP combined waste molasses reinforced calcareous sand. The preparation method comprises the following steps: carrying out activation culture on mineralized bacteria, preparing a waste molasses culture medium by using waste molasses, inoculating activated mineralized bacteria liquid on the waste molasses culture medium, culturing until the OD600 value of the mineralized bacteria in the waste molasses culture medium is 1.0-1.5, injecting the waste molasses culture medium containing the mineralized bacteria into calcareous sand for reinforcement, and carrying out vacuum drying on the calcareous sand. The reinforced calcareous sand is obtained. According to the preparation method of the MICP combined waste molasses reinforced calcareous sand, the waste molasses is directly used as a carbon source to culture mineralized strains, so that the preparation cost is greatly reduced, and the preparation process is green and environment-friendly; the bagasse with a proper proportion and a proper length is added into the waste molasses, so that the viscous sugar filaments and the bagasse are uniformly distributed in the reinforced calcareous sand, the generation amount of calcium carbonate crystals induced by microorganisms is increased, the prepared calcareous sand has a more excellent reinforcing effect, and the maximum unconfined compressive strength can reach 2MPa.
本发明公开了一种MICP联合废糖蜜加固钙质砂的制备方法。所述制备方法包括以下步骤：将矿化菌进行活化培养，利用废糖蜜制备废糖蜜培养基，将活化后的矿化菌菌液接种于废糖蜜培养基上，培养至废糖蜜培养基中矿化菌的OD600值在1.0～1.5之间后，将含有矿化菌的废糖蜜培养基注入钙质砂中进行加固，即得加固钙质砂。本发明公开的MICP联合废糖蜜加固钙质砂的制备方法，利用废糖蜜直接作为碳源培养矿化菌种，大幅降低制备成本，且制备过程绿色环保；通过在废糖蜜中添加适当占比及长度的甘蔗渣，使粘稠的糖丝和甘蔗渣均匀分布在加固后的钙质砂中，从而提高微生物诱导的碳酸钙晶体的生成量，使制备得到的钙质砂具有更加优异的加固效果，无侧限抗压强度最高可达2MPa。
Preparation method of MICP combined waste molasses reinforced calcareous sand
The invention discloses a preparation method of MICP combined waste molasses reinforced calcareous sand. The preparation method comprises the following steps: carrying out activation culture on mineralized bacteria, preparing a waste molasses culture medium by using waste molasses, inoculating activated mineralized bacteria liquid on the waste molasses culture medium, culturing until the OD600 value of the mineralized bacteria in the waste molasses culture medium is 1.0-1.5, injecting the waste molasses culture medium containing the mineralized bacteria into calcareous sand for reinforcement, and carrying out vacuum drying on the calcareous sand. The reinforced calcareous sand is obtained. According to the preparation method of the MICP combined waste molasses reinforced calcareous sand, the waste molasses is directly used as a carbon source to culture mineralized strains, so that the preparation cost is greatly reduced, and the preparation process is green and environment-friendly; the bagasse with a proper proportion and a proper length is added into the waste molasses, so that the viscous sugar filaments and the bagasse are uniformly distributed in the reinforced calcareous sand, the generation amount of calcium carbonate crystals induced by microorganisms is increased, the prepared calcareous sand has a more excellent reinforcing effect, and the maximum unconfined compressive strength can reach 2MPa.
本发明公开了一种MICP联合废糖蜜加固钙质砂的制备方法。所述制备方法包括以下步骤：将矿化菌进行活化培养，利用废糖蜜制备废糖蜜培养基，将活化后的矿化菌菌液接种于废糖蜜培养基上，培养至废糖蜜培养基中矿化菌的OD600值在1.0～1.5之间后，将含有矿化菌的废糖蜜培养基注入钙质砂中进行加固，即得加固钙质砂。本发明公开的MICP联合废糖蜜加固钙质砂的制备方法，利用废糖蜜直接作为碳源培养矿化菌种，大幅降低制备成本，且制备过程绿色环保；通过在废糖蜜中添加适当占比及长度的甘蔗渣，使粘稠的糖丝和甘蔗渣均匀分布在加固后的钙质砂中，从而提高微生物诱导的碳酸钙晶体的生成量，使制备得到的钙质砂具有更加优异的加固效果，无侧限抗压强度最高可达2MPa。
Preparation method of MICP combined waste molasses reinforced calcareous sand
一种MICP联合废糖蜜加固钙质砂的制备方法
MA BIN (author) / WANG LIYAN (author) / ZHU MINGXING (author) / ZHAN QIWEI (author) / LI XIAOJUAN (author) / LIU SHUNQING (author) / QI YONGZHENG (author) / FANG ZHIKAI (author) / TAN BORUI (author) / JIANG MINGRUI (author)
2023-12-29
Patent
Electronic Resource
Chinese
| European Patent Office | 2022
Particle Morphology of Calcareous Sand and MICP-Treated Efficiency
| Springer Verlag | 2024
Study on calcareous sand treated by MICP in different NaCl concentrations
| Taylor & Francis Verlag | 2023
Liquefaction resistance of MICP treated silica and calcareous sand with carbon fiber
| Springer Verlag | 2025