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Electrochemical single nucleotide polymorphism detection using hoechst 33258 groove binder
Single Nucleotide Polymorphisms (SNPs) analysis requires a low cost detection technology that is capable of miniaturization, multiplexing, and high sensitivity. In this research, a DNA chip with a microelectrode array was fabricated using microfabrication technology. Several probe DNAs consisting of mercaptohexyl moiety at their 5’ end were immobilized on gold electrodes by using a DNA arrayer. Then, target DNAs were hybridized and reacted with Hoechst 33258, which is a DNA minor groove binder and electrochemically active dye. Linear sweep voltammetry or cyclic voltammetry showed a difference in the anodic peak current values between target DNA, mismatched DNA, and control DNA.
Electrochemical single nucleotide polymorphism detection using hoechst 33258 groove binder
Single Nucleotide Polymorphisms (SNPs) analysis requires a low cost detection technology that is capable of miniaturization, multiplexing, and high sensitivity. In this research, a DNA chip with a microelectrode array was fabricated using microfabrication technology. Several probe DNAs consisting of mercaptohexyl moiety at their 5’ end were immobilized on gold electrodes by using a DNA arrayer. Then, target DNAs were hybridized and reacted with Hoechst 33258, which is a DNA minor groove binder and electrochemically active dye. Linear sweep voltammetry or cyclic voltammetry showed a difference in the anodic peak current values between target DNA, mismatched DNA, and control DNA.
Electrochemical single nucleotide polymorphism detection using hoechst 33258 groove binder
Yong-Sung Choi, (author) / Young-Soo Kwon, (author) / Kyung-Sup Lee, (author)
2006-10-01
419990 byte
Conference paper
Electronic Resource
English
Single Nucleotide Polymorphism Genotyping in Single‐Molecule Electronic Circuits
| Wiley | 2017
| UB Braunschweig | 1970
| TIBKAT | 1970
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