A platform for research: civil engineering, architecture and urbanism
A platform for research: civil engineering, architecture and urbanism
Optimization study on extraction of antioxidants from plum seeds (Prunus domestica L.)
This paper aimed to optimize the extraction of antioxidants from plum seeds (Prunus domestica L.) using ultrasound-assisted extraction. The Box–Behnken design was used for the optimization of the extraction process. The four extraction parameters, such as the extraction time (10–40 min), ethanol concentration (20–100%, v/v), liquid-to-solid ratio (10–30 cm3 g−1), and extraction temperature (30–70 °C) were varied to investigate their impact on the content of antioxidants. Using HPLC methods, the following phenolic compounds were identified and quantified per 100 g dry weight: rutin (6.39 mg), epigallocatechin (1.94 mg), gallic acid (0.64 mg), ferulic acid (14.30 mg), syringic acid (0.87 mg), epicatechin (0.95 mg), caffeic acid (0.30 mg), and coumaric acid (11.18 mg). The content of amygdalin was 1.5 mg g−1 of the dry extract obtained under optimal conditions. Antioxidant activity was determined using the DPPH assay and estimated based on the half-maximal inhibitory concentration (IC50). The IC50 value of the extract obtained under optimal conditions was 0.94 mg cm−3. The proposed green technique is an economical and alternative procedure for the extraction of antioxidants by application of non-toxic and eco-friendly solvents. From the ecological point of view, it is acceptable due to the reduction of waste either after plum cultivation or production of alcoholic beverages.
Optimization study on extraction of antioxidants from plum seeds (Prunus domestica L.)
This paper aimed to optimize the extraction of antioxidants from plum seeds (Prunus domestica L.) using ultrasound-assisted extraction. The Box–Behnken design was used for the optimization of the extraction process. The four extraction parameters, such as the extraction time (10–40 min), ethanol concentration (20–100%, v/v), liquid-to-solid ratio (10–30 cm3 g−1), and extraction temperature (30–70 °C) were varied to investigate their impact on the content of antioxidants. Using HPLC methods, the following phenolic compounds were identified and quantified per 100 g dry weight: rutin (6.39 mg), epigallocatechin (1.94 mg), gallic acid (0.64 mg), ferulic acid (14.30 mg), syringic acid (0.87 mg), epicatechin (0.95 mg), caffeic acid (0.30 mg), and coumaric acid (11.18 mg). The content of amygdalin was 1.5 mg g−1 of the dry extract obtained under optimal conditions. Antioxidant activity was determined using the DPPH assay and estimated based on the half-maximal inhibitory concentration (IC50). The IC50 value of the extract obtained under optimal conditions was 0.94 mg cm−3. The proposed green technique is an economical and alternative procedure for the extraction of antioxidants by application of non-toxic and eco-friendly solvents. From the ecological point of view, it is acceptable due to the reduction of waste either after plum cultivation or production of alcoholic beverages.
Optimization study on extraction of antioxidants from plum seeds (Prunus domestica L.)
Optim Eng
Savic, Ivan M. (author) / Savic Gajic, Ivana M. (author)
Optimization and Engineering ; 22 ; 141-158
2021-03-01
18 pages
Article (Journal)
Electronic Resource
English
Natural fat antioxidants in seeds of selected plants
| British Library Online Contents | 1994