A platform for research: civil engineering, architecture and urbanism
A platform for research: civil engineering, architecture and urbanism
In situ biomass flocculation improves placement of Sporosarcina Pasteurii for microbially mediated sandy soil stabilization
https://orcid.org/http://orcid.org/0000-0002-1767-2108
Microbially induced carbonate precipitates (MICPs) through ureolysis-driven calcite precipitation have been investigated as a mean of improving the mechanical properties of soil (cohesion, friction, stiffness, and permeability). To achieve a well-controlled and uniform MICP, it is crucial to obtain a homogeneous distribution of bacterial activity. This paper describes a new and simple method to maximize the retention of bacteria in porous silica sand, where the retained bacteria with their activity are distributed homogeneously. This method is based on a novel in situ biomass flocculation technique induced by the presence of a trace amount of Ca2+ and increased alkalinity of the environment due to the hydrolysis of urea. The method has been tested in both 300 mm and 1000 mm sand columns, in which the retained urease activity, content of the produced CaCO3, and final unconfined compressive strength were homogeneously distributed throughout the entire treated columns. The presented bacterial fixation method could also be potentially used to deliver and fix specific bacteria in a target zone. Overall, this method of improving bacterial fixation in porous media can be used for bio-cementation ground improvement, ensuring the uniform performance of treated soils.
In situ biomass flocculation improves placement of Sporosarcina Pasteurii for microbially mediated sandy soil stabilization
https://orcid.org/http://orcid.org/0000-0002-1767-2108
Microbially induced carbonate precipitates (MICPs) through ureolysis-driven calcite precipitation have been investigated as a mean of improving the mechanical properties of soil (cohesion, friction, stiffness, and permeability). To achieve a well-controlled and uniform MICP, it is crucial to obtain a homogeneous distribution of bacterial activity. This paper describes a new and simple method to maximize the retention of bacteria in porous silica sand, where the retained bacteria with their activity are distributed homogeneously. This method is based on a novel in situ biomass flocculation technique induced by the presence of a trace amount of Ca2+ and increased alkalinity of the environment due to the hydrolysis of urea. The method has been tested in both 300 mm and 1000 mm sand columns, in which the retained urease activity, content of the produced CaCO3, and final unconfined compressive strength were homogeneously distributed throughout the entire treated columns. The presented bacterial fixation method could also be potentially used to deliver and fix specific bacteria in a target zone. Overall, this method of improving bacterial fixation in porous media can be used for bio-cementation ground improvement, ensuring the uniform performance of treated soils.
In situ biomass flocculation improves placement of Sporosarcina Pasteurii for microbially mediated sandy soil stabilization
https://orcid.org/http://orcid.org/0000-0002-1767-2108
Microbially induced carbonate precipitates (MICPs) through ureolysis-driven calcite precipitation have been investigated as a mean of improving the mechanical properties of soil (cohesion, friction, stiffness, and permeability). To achieve a well-controlled and uniform MICP, it is crucial to obtain a homogeneous distribution of bacterial activity. This paper describes a new and simple method to maximize the retention of bacteria in porous silica sand, where the retained bacteria with their activity are distributed homogeneously. This method is based on a novel in situ biomass flocculation technique induced by the presence of a trace amount of Ca2+ and increased alkalinity of the environment due to the hydrolysis of urea. The method has been tested in both 300 mm and 1000 mm sand columns, in which the retained urease activity, content of the produced CaCO3, and final unconfined compressive strength were homogeneously distributed throughout the entire treated columns. The presented bacterial fixation method could also be potentially used to deliver and fix specific bacteria in a target zone. Overall, this method of improving bacterial fixation in porous media can be used for bio-cementation ground improvement, ensuring the uniform performance of treated soils.
In situ biomass flocculation improves placement of Sporosarcina Pasteurii for microbially mediated sandy soil stabilization
Acta Geotech.
Yang, Yang (author) / Xiao, Yang (author) / Cheng, Liang (author) / Shahin, Mohamed A. (author) / Liu, Hanlong (author)
Acta Geotechnica ; 17 ; 4435-4445
2022-10-01
11 pages
Article (Journal)
Electronic Resource
English
Bio-cementation , Ground improvement , Soil stabilization , Microbially induced carbonate precipitation (MICP) Engineering , Geoengineering, Foundations, Hydraulics , Solid Mechanics , Geotechnical Engineering & Applied Earth Sciences , Soil Science & Conservation , Soft and Granular Matter, Complex Fluids and Microfluidics
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