A platform for research: civil engineering, architecture and urbanism
A platform for research: civil engineering, architecture and urbanism
Modified SICP method to mitigate the effect of bio-clogging by excess protein from soybean crude urease extracts for biocementation process
Enzyme-induced carbonate precipitation (EICP) is an emerging soil improvement technique to precipitate in situ calcite carbonate to cement soil particles together via free urease. Soybean-derived urease is a potential alternative to commercial urease which could be applied in the large-scale application in practical engineering of EICP process because of its low cost. However, the soybean crude urease extracted by common methods contains various excess proteins, hindering the biocementation process. In this paper, the adverse effect of excess proteins in soybean crude urease was studied, and a modified soybean urease-induced carbonate precipitation (SICP) method is proposed to mitigate that adverse effect during the biocementation process. The test results have shown that the excess protein in soybean crude urease may lead to bio-clogging problems which result in non-uniform CaCO3 precipitation distribution and low UCS during the biocementation process. Furthermore, the modified SICP method can effectively mitigate the adverse impact of excess proteins and significantly improve the biocementation effect as compared with the conventional method.
Modified SICP method to mitigate the effect of bio-clogging by excess protein from soybean crude urease extracts for biocementation process
Enzyme-induced carbonate precipitation (EICP) is an emerging soil improvement technique to precipitate in situ calcite carbonate to cement soil particles together via free urease. Soybean-derived urease is a potential alternative to commercial urease which could be applied in the large-scale application in practical engineering of EICP process because of its low cost. However, the soybean crude urease extracted by common methods contains various excess proteins, hindering the biocementation process. In this paper, the adverse effect of excess proteins in soybean crude urease was studied, and a modified soybean urease-induced carbonate precipitation (SICP) method is proposed to mitigate that adverse effect during the biocementation process. The test results have shown that the excess protein in soybean crude urease may lead to bio-clogging problems which result in non-uniform CaCO3 precipitation distribution and low UCS during the biocementation process. Furthermore, the modified SICP method can effectively mitigate the adverse impact of excess proteins and significantly improve the biocementation effect as compared with the conventional method.
Modified SICP method to mitigate the effect of bio-clogging by excess protein from soybean crude urease extracts for biocementation process
Acta Geotech.
Yan, Boyang (author) / Zhou, Yundong (author) / Li, Chi (author) / Shu, Shuang (author) / Gao, Yufeng (author)
Acta Geotechnica ; 18 ; 5047-5062
2023-09-01
16 pages
Article (Journal)
Electronic Resource
English
Biocementation , Bio-clogging , Soil improvement , Soybean urease-induced carbonate precipitation (SICP) Engineering , Geoengineering, Foundations, Hydraulics , Solid Mechanics , Geotechnical Engineering & Applied Earth Sciences , Soil Science & Conservation , Soft and Granular Matter, Complex Fluids and Microfluidics