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Quantification of Bacteria Self-healing Efficiency on Concrete Cracks
Use of a biological repair technique such as Microbial Induced Calcite Precipitation (MICP) has proven its success by previous research works. Previous studies concluded each bacteria has different healing capability depending on its population rate and width of crack to be healed. In this study, Sporosarcina pasteurii (S. pasteurii) was prepared in NH4YE media with additional of 5 g/L of filter sterilised urea solution. Three (3) series of specimens with artificial cracks were used for the application of bacteria taken from three (3) different highest population rate onto the cracks and one (1) control cracked specimen without bacteria. The number of live cells in the S. pasteurii population over a period of time was examined at hour 24, 33 and 48. Bacterial cell concentrations consisted of 207 × 106 cfu/ml at hour 24 (Specimen 1), 835 × 106 cfu/ml at hour 33 (Specimen 2) and 2.88 × 109 cfu/ml at hour 48 (Specimen 3) were dropped into the crack created on each specimen by using a 1 ml pipette/dropper. The formation of filling material was verified using Scanning Electron Microscopy (SEM). The results indicated that the crack created on Specimen 3 which was injected with bacteria concentration of 2.88 × 109 cfu/ml taken at highest population rate growth exhibited highest healing efficiency (HE). The crack was 100% fully healed after 28 days applying the bacteria broth solution. Meanwhile, it showed that those with higher bacteria concentration marked higher healing efficiency capacity. Calcium carbonate (calcite) formation as the crack filling material was confirmed by SEM examination. The results indicated that S. pasteurii in broth form has great potential to be used as crack remediation agent in concrete.
Quantification of Bacteria Self-healing Efficiency on Concrete Cracks
Use of a biological repair technique such as Microbial Induced Calcite Precipitation (MICP) has proven its success by previous research works. Previous studies concluded each bacteria has different healing capability depending on its population rate and width of crack to be healed. In this study, Sporosarcina pasteurii (S. pasteurii) was prepared in NH4YE media with additional of 5 g/L of filter sterilised urea solution. Three (3) series of specimens with artificial cracks were used for the application of bacteria taken from three (3) different highest population rate onto the cracks and one (1) control cracked specimen without bacteria. The number of live cells in the S. pasteurii population over a period of time was examined at hour 24, 33 and 48. Bacterial cell concentrations consisted of 207 × 106 cfu/ml at hour 24 (Specimen 1), 835 × 106 cfu/ml at hour 33 (Specimen 2) and 2.88 × 109 cfu/ml at hour 48 (Specimen 3) were dropped into the crack created on each specimen by using a 1 ml pipette/dropper. The formation of filling material was verified using Scanning Electron Microscopy (SEM). The results indicated that the crack created on Specimen 3 which was injected with bacteria concentration of 2.88 × 109 cfu/ml taken at highest population rate growth exhibited highest healing efficiency (HE). The crack was 100% fully healed after 28 days applying the bacteria broth solution. Meanwhile, it showed that those with higher bacteria concentration marked higher healing efficiency capacity. Calcium carbonate (calcite) formation as the crack filling material was confirmed by SEM examination. The results indicated that S. pasteurii in broth form has great potential to be used as crack remediation agent in concrete.
Quantification of Bacteria Self-healing Efficiency on Concrete Cracks
Lecture Notes in Civil Engineering
Belayutham, Sheila (editor) / Che Ibrahim, Che Khairil Izam (editor) / Alisibramulisi, Anizahyati (editor) / Mansor, Hazrina (editor) / Billah, Muntasir (editor) / Hamidah, M. S. (author) / Noor Hana, H. (author) / Iqmal, K. M. G. (author) / Kartini, K. (author)
International Conference on Sustainable Civil Engineering Structures and Construction Materials ; 2020
2022-04-07
15 pages
Article/Chapter (Book)
Electronic Resource
English
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