A platform for research: civil engineering, architecture and urbanism
A platform for research: civil engineering, architecture and urbanism
Detection of Escherichia coli in wastewater based on enzyme immunoassay
Abstract This research describes a fast detection method on the basis of enzyme-linked immunosorbent assay (ELISA) for Escherichia coli in drainage of wastewater treatment plants. Optimized conditions such as the reaction format (sandwich or direct), the concentrations of diluted horseradish peroxidase (HRP)-E. coli conjugate, and anti-HPR antibody and pretreatment of E. coli were studied. Those results showed that the linear range of detection for E. coli was 10 cfu/mL-6 × 104 cfu/mL. Compared with conventional methods, it is a convenient and sensitive detection method with low cost.
Detection of Escherichia coli in wastewater based on enzyme immunoassay
Abstract This research describes a fast detection method on the basis of enzyme-linked immunosorbent assay (ELISA) for Escherichia coli in drainage of wastewater treatment plants. Optimized conditions such as the reaction format (sandwich or direct), the concentrations of diluted horseradish peroxidase (HRP)-E. coli conjugate, and anti-HPR antibody and pretreatment of E. coli were studied. Those results showed that the linear range of detection for E. coli was 10 cfu/mL-6 × 104 cfu/mL. Compared with conventional methods, it is a convenient and sensitive detection method with low cost.
Detection of Escherichia coli in wastewater based on enzyme immunoassay
Xi, Haiyan (author) / Cai, Qiang (author) / He, Miao (author) / Shi, Hanchang (author)
2007-07-01
4 pages
Article (Journal)
Electronic Resource
English
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