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Rapid identification of Streptomyces tetracycline producers by MALDI-TOF mass spectrometry
The main objective of this study was using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for assembling of DSM (German Collection of Microorganisms) Streptomyces spectral database and identification of wild Streptomyces cultures, which were clustered by MALDI-TOF Biotyper OC software as well as for teracycline detection by observing of obtained spectra using flexAnalysis software. Production of tetracycline was confirmed by thin-layer chromatography. Presence of tetracycline mass spectrum was verified by several tetracycline producers (Streptomyces aureofaciens LMG 5968, S. aureofaciens 84/25, and S. aureofaciens BMK) and by pure tetracycline mass. Our results showed that it is possible to use MALDI-TOF MS for identification of tetracycline producers within Streptomyces genera by several easy steps. The purpose of this study was to establish cheap and quick detection of tetracycline producers.
Rapid identification of Streptomyces tetracycline producers by MALDI-TOF mass spectrometry
The main objective of this study was using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for assembling of DSM (German Collection of Microorganisms) Streptomyces spectral database and identification of wild Streptomyces cultures, which were clustered by MALDI-TOF Biotyper OC software as well as for teracycline detection by observing of obtained spectra using flexAnalysis software. Production of tetracycline was confirmed by thin-layer chromatography. Presence of tetracycline mass spectrum was verified by several tetracycline producers (Streptomyces aureofaciens LMG 5968, S. aureofaciens 84/25, and S. aureofaciens BMK) and by pure tetracycline mass. Our results showed that it is possible to use MALDI-TOF MS for identification of tetracycline producers within Streptomyces genera by several easy steps. The purpose of this study was to establish cheap and quick detection of tetracycline producers.
Rapid identification of Streptomyces tetracycline producers by MALDI-TOF mass spectrometry
Hleba, Lukas (author) / Charousova, Ivana (author) / Cisarova, Miroslava (author) / Kovacik, Anton (author) / Kormanec, Jan (author) / Medo, Juraj (author) / Bozik, Matej (author) / Javorekova, Sona (author)
Journal of Environmental Science and Health, Part A ; 53 ; 1083-1093
2018-10-15
11 pages
Article (Journal)
Electronic Resource
English
Identification of Streptomyces Isolated from Environmental Soil Samples Using Rapid Enzyme Data
| Online Contents | 1995
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| Taylor & Francis Verlag | 2018