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Submerged filter biofilm formation by nitrate-contaminated groundwater microbiota
Denitrifying biofilms developed in a lab-scale submerged filter by autochthonous bacteria from nitrate-contaminated groundwater were studied. The system was supplied with groundwater (16 mg N-NO3-/L), from which the oxygen had been eliminated and to which an excess of carbon source had been added. The reactor was incubated in a thermostated chamber at 5°C, 10°C, 20°C and 30°C. Colonization of the support was studied using surface scanning microscopy, and biofilm bacterial composition was studied by PCR/TGGE. Support material was colonized at all the temperatures assayed, although this parameter affected the growth of the biofilm, which developed most at temperatures over 20°C. The composition of bacterial communities varied according to the temperature. Community profiles of the biofilm formed at 5°C and 10°C clustered away from those of the biofilm formed at 20°C and 30°C. 16S rDNA sequences reveled that the biofilm was mainly composed of psychrotolerant species typically inhabiting freshwaters, and we obtained sequencing bands that were affiliated to denitrifying and non-denitrifying heterotrophic species. The extent of colonization was low when compared to previously inoculated systems, and the capacity for nitrate elimination was also low.
Submerged filter biofilm formation by nitrate-contaminated groundwater microbiota
Denitrifying biofilms developed in a lab-scale submerged filter by autochthonous bacteria from nitrate-contaminated groundwater were studied. The system was supplied with groundwater (16 mg N-NO3-/L), from which the oxygen had been eliminated and to which an excess of carbon source had been added. The reactor was incubated in a thermostated chamber at 5°C, 10°C, 20°C and 30°C. Colonization of the support was studied using surface scanning microscopy, and biofilm bacterial composition was studied by PCR/TGGE. Support material was colonized at all the temperatures assayed, although this parameter affected the growth of the biofilm, which developed most at temperatures over 20°C. The composition of bacterial communities varied according to the temperature. Community profiles of the biofilm formed at 5°C and 10°C clustered away from those of the biofilm formed at 20°C and 30°C. 16S rDNA sequences reveled that the biofilm was mainly composed of psychrotolerant species typically inhabiting freshwaters, and we obtained sequencing bands that were affiliated to denitrifying and non-denitrifying heterotrophic species. The extent of colonization was low when compared to previously inoculated systems, and the capacity for nitrate elimination was also low.
Submerged filter biofilm formation by nitrate-contaminated groundwater microbiota
de la Rua, A. (author) / Rodelas, B. (author) / Gonzalez-Lopez, J. (author) / Gomez, M. A. (author)
Journal of Environmental Science and Health, Part A ; 46 ; 1113-1121
2011-08-01
9 pages
Article (Journal)
Electronic Resource
English
Submerged filter biofilm formation by nitrate-contaminated groundwater microbiota
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