A platform for research: civil engineering, architecture and urbanism
A platform for research: civil engineering, architecture and urbanism
Multidirectional Filamented Light Biofabrication Creates Aligned and Contractile Cardiac Tissues
https://orcid.org/0000-0003-0381-2285
https://orcid.org/0000-0002-9651-406X
https://orcid.org/0000-0002-5291-0255
https://orcid.org/0000-0001-9645-9868
https://orcid.org/0000-0002-0282-6127
https://orcid.org/0000-0001-5124-1195
https://orcid.org/0000-0002-4994-1691
https://orcid.org/0000-0001-8396-3256
https://orcid.org/0000-0002-5212-9426
https://orcid.org/0000-0001-7143-7259
AbstractBiofabricating 3D cardiac tissues that mimic the native myocardial tissue is a pivotal challenge in tissue engineering. In this study, we fabricate 3D cardiac tissues with controlled, multidirectional cellular alignment and directed or twisting contractility. We show that multidirectional filamented light can be used to biofabricate high‐density (up to 60 × 106 cells mL−1) tissues, with directed uniaxial contractility (3.8x) and improved cell‐to‐cell connectivity (1.6x gap junction expression). Furthermore, by using multidirectional light projection, we can partially overcome cell‐induced light attenuation, and fabricate larger tissues with multidirectional cellular alignment. For example, we fabricate a tri‐layered myocardium‐like tissue and a bi‐layered tissue with torsional contractility. The approach provides a new strategy to rapidly fabricate aligned cardiac tissues relevant to regenerative medicine and biohybrid robotics.
Multidirectional Filamented Light Biofabrication Creates Aligned and Contractile Cardiac Tissues
https://orcid.org/0000-0003-0381-2285
https://orcid.org/0000-0002-9651-406X
https://orcid.org/0000-0002-5291-0255
https://orcid.org/0000-0001-9645-9868
https://orcid.org/0000-0002-0282-6127
https://orcid.org/0000-0001-5124-1195
https://orcid.org/0000-0002-4994-1691
https://orcid.org/0000-0001-8396-3256
https://orcid.org/0000-0002-5212-9426
https://orcid.org/0000-0001-7143-7259
AbstractBiofabricating 3D cardiac tissues that mimic the native myocardial tissue is a pivotal challenge in tissue engineering. In this study, we fabricate 3D cardiac tissues with controlled, multidirectional cellular alignment and directed or twisting contractility. We show that multidirectional filamented light can be used to biofabricate high‐density (up to 60 × 106 cells mL−1) tissues, with directed uniaxial contractility (3.8x) and improved cell‐to‐cell connectivity (1.6x gap junction expression). Furthermore, by using multidirectional light projection, we can partially overcome cell‐induced light attenuation, and fabricate larger tissues with multidirectional cellular alignment. For example, we fabricate a tri‐layered myocardium‐like tissue and a bi‐layered tissue with torsional contractility. The approach provides a new strategy to rapidly fabricate aligned cardiac tissues relevant to regenerative medicine and biohybrid robotics.
Multidirectional Filamented Light Biofabrication Creates Aligned and Contractile Cardiac Tissues
https://orcid.org/0000-0003-0381-2285
https://orcid.org/0000-0002-9651-406X
https://orcid.org/0000-0002-5291-0255
https://orcid.org/0000-0001-9645-9868
https://orcid.org/0000-0002-0282-6127
https://orcid.org/0000-0001-5124-1195
https://orcid.org/0000-0002-4994-1691
https://orcid.org/0000-0001-8396-3256
https://orcid.org/0000-0002-5212-9426
https://orcid.org/0000-0001-7143-7259
AbstractBiofabricating 3D cardiac tissues that mimic the native myocardial tissue is a pivotal challenge in tissue engineering. In this study, we fabricate 3D cardiac tissues with controlled, multidirectional cellular alignment and directed or twisting contractility. We show that multidirectional filamented light can be used to biofabricate high‐density (up to 60 × 106 cells mL−1) tissues, with directed uniaxial contractility (3.8x) and improved cell‐to‐cell connectivity (1.6x gap junction expression). Furthermore, by using multidirectional light projection, we can partially overcome cell‐induced light attenuation, and fabricate larger tissues with multidirectional cellular alignment. For example, we fabricate a tri‐layered myocardium‐like tissue and a bi‐layered tissue with torsional contractility. The approach provides a new strategy to rapidly fabricate aligned cardiac tissues relevant to regenerative medicine and biohybrid robotics.
Multidirectional Filamented Light Biofabrication Creates Aligned and Contractile Cardiac Tissues
Advanced Science
Jones, Lewis S. (author) / Filippi, Miriam (author) / Michelis, Mike Yan (author) / Balciunaite, Aiste (author) / Yasa, Oncay (author) / Aviel, Gal (author) / Narciso, Maria (author) / Freedrich, Susanne (author) / Generali, Melanie (author) / Tzahor, Eldad (author)
Advanced Science ; 11
2024-12-01
Article (Journal)
Electronic Resource
English
Use of Filamented Beam Elements for Bored Pile Analysis
| British Library Online Contents | 1997
Use of Filamented Beam Elements for Bored Pile Analysis
| Online Contents | 1997
Matrix-assisted pulsed laser methods for biofabrication
| British Library Online Contents | 2011
CONTINUOUS MULTIDIRECTIONAL ULTRA-LIGHT ULTRA-STRONG STRUCTURE
| European Patent Office | 2016