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In vivoandin vitroliver and gill EROD activity in rainbow trout (Oncorhynchus mykiss) exposed to the beta‐blocker propranolol
AbstractThe conservation of common physiological systems across vertebrate classes suggests the potential for certain pharmaceuticals, which have been detected in surface waters, to produce biological effects in nontarget vertebrates such as fish. However, previous studies assessing the effects of such compounds in fish have not taken into account the potential for metabolism and elimination. This study aimed to assess if propranolol, a β‐adrenergic receptor antagonist or β‐blocker, could modulate EROD activity (indicative of CYP1A activity) in rainbow trout (Oncorhynchus mykiss) gills and liver. For this, anin vivotime course exposure with 1 mg/L was conducted. Additionally, using measuredin vivoplasma concentrations, anin vitroexposure at human therapeutic levels was undertaken. This allowed comparison ofin vitroandin vivorates of EROD activity, thus investigating the applicability of cell preparations as surrogates for whole animal enzyme activity analysis.In vitroexposure of suspended liver and gill cells at concentrations similar toin vivolevels resulted in EROD activity in both tissues, but with significantly higher rates (up to six timesin vivolevels). These results show that propranolol exposure elevated EROD activity in the liver and gill of rainbow trout, and that this is demonstrable bothin vivo(albeit nonsignificantly in the liver) andin vitro, thus supporting the use of the latter as a surrogate of the former. These data also provide an insight into the potential role of the gill as a site of metabolism of pharmaceuticals in trout, suggesting that propranolol (and feasibly other pharmaceuticals) may undergo “first pass” metabolism in this organ. © 2011 Wiley Periodicals, Inc. Environ Toxicol 2012.
In vivoandin vitroliver and gill EROD activity in rainbow trout (Oncorhynchus mykiss) exposed to the beta‐blocker propranolol
AbstractThe conservation of common physiological systems across vertebrate classes suggests the potential for certain pharmaceuticals, which have been detected in surface waters, to produce biological effects in nontarget vertebrates such as fish. However, previous studies assessing the effects of such compounds in fish have not taken into account the potential for metabolism and elimination. This study aimed to assess if propranolol, a β‐adrenergic receptor antagonist or β‐blocker, could modulate EROD activity (indicative of CYP1A activity) in rainbow trout (Oncorhynchus mykiss) gills and liver. For this, anin vivotime course exposure with 1 mg/L was conducted. Additionally, using measuredin vivoplasma concentrations, anin vitroexposure at human therapeutic levels was undertaken. This allowed comparison ofin vitroandin vivorates of EROD activity, thus investigating the applicability of cell preparations as surrogates for whole animal enzyme activity analysis.In vitroexposure of suspended liver and gill cells at concentrations similar toin vivolevels resulted in EROD activity in both tissues, but with significantly higher rates (up to six timesin vivolevels). These results show that propranolol exposure elevated EROD activity in the liver and gill of rainbow trout, and that this is demonstrable bothin vivo(albeit nonsignificantly in the liver) andin vitro, thus supporting the use of the latter as a surrogate of the former. These data also provide an insight into the potential role of the gill as a site of metabolism of pharmaceuticals in trout, suggesting that propranolol (and feasibly other pharmaceuticals) may undergo “first pass” metabolism in this organ. © 2011 Wiley Periodicals, Inc. Environ Toxicol 2012.
In vivoandin vitroliver and gill EROD activity in rainbow trout (Oncorhynchus mykiss) exposed to the beta‐blocker propranolol
Environmental Toxicology
Bartram, Abigail E. (author) / Winter, Matthew J. (author) / Huggett, Duane B. (author) / McCormack, Paul (author) / Constantine, Lisa A. (author) / Hetheridge, Malcolm J. (author) / Hutchinson, Thomas H. (author) / Kinter, Lewis B. (author) / Ericson, Jon. F. (author) / Sumpter, John P. (author)
Environmental Toxicology ; 27 ; 573-582
2012-10-01
Article (Journal)
Electronic Resource
English
| British Library Conference Proceedings | 1996
| British Library Online Contents | 2010