A platform for research: civil engineering, architecture and urbanism
A platform for research: civil engineering, architecture and urbanism
X‐Linked Gene Dosage and SOX2 Act as Key Roadblocks for Human Germ Cell Specification in Klinefelter Syndrome
https://orcid.org/0000-0002-3549-7593
https://orcid.org/0000-0001-5934-7816
AbstractKlinefelter syndrome (KS), characterized by the presence of at least one extra X‐chromosome, is a common cause of male infertility. However, the mechanism underlying the failure of germline specification is not well studied. Intriguingly, the differentiation efficiency of female human pluripotent stem cells (hPSCs) is often lower than that of male. This study investigates how X‐linked gene dosage affects human primordial germ cell‐like cells (hPGCLCs) specification in both healthy and diseased conditions. This work reveals that X‐linked genes play a multifaceted role against the fate competency to hPGCLCs, with escape genes IGSF1 and CHRDL1 inhibiting the TGF‐beta/Activin A and BMP pathways, respectively. Notably, this work identifies a previously unrecognized role of SOX2, upregulated by the escape gene USP9X, elucidating a species‐specific function in the mammalian germline. The USP9X‐SOX2 regulatory axis profoundly influenced cellular metabolism, mitochondrial morphology, and progenitor competence in hPGCLCs specification. Furthermore, the inability to downregulate SOX2 and upregulate SOX17 in response to BMP signaling impedes downstream gene activation due to motif binding competition. These findings shed novel insights into the human germline specification by elucidating the divergent roles of SOX2 versus SOX17 in mammals, influenced by X‐linked gene dosage effects. These results offer potential applications for improving the induction efficiency of hPGCLCs, facilitating disease mechanistic studies.
X‐Linked Gene Dosage and SOX2 Act as Key Roadblocks for Human Germ Cell Specification in Klinefelter Syndrome
https://orcid.org/0000-0002-3549-7593
https://orcid.org/0000-0001-5934-7816
AbstractKlinefelter syndrome (KS), characterized by the presence of at least one extra X‐chromosome, is a common cause of male infertility. However, the mechanism underlying the failure of germline specification is not well studied. Intriguingly, the differentiation efficiency of female human pluripotent stem cells (hPSCs) is often lower than that of male. This study investigates how X‐linked gene dosage affects human primordial germ cell‐like cells (hPGCLCs) specification in both healthy and diseased conditions. This work reveals that X‐linked genes play a multifaceted role against the fate competency to hPGCLCs, with escape genes IGSF1 and CHRDL1 inhibiting the TGF‐beta/Activin A and BMP pathways, respectively. Notably, this work identifies a previously unrecognized role of SOX2, upregulated by the escape gene USP9X, elucidating a species‐specific function in the mammalian germline. The USP9X‐SOX2 regulatory axis profoundly influenced cellular metabolism, mitochondrial morphology, and progenitor competence in hPGCLCs specification. Furthermore, the inability to downregulate SOX2 and upregulate SOX17 in response to BMP signaling impedes downstream gene activation due to motif binding competition. These findings shed novel insights into the human germline specification by elucidating the divergent roles of SOX2 versus SOX17 in mammals, influenced by X‐linked gene dosage effects. These results offer potential applications for improving the induction efficiency of hPGCLCs, facilitating disease mechanistic studies.
X‐Linked Gene Dosage and SOX2 Act as Key Roadblocks for Human Germ Cell Specification in Klinefelter Syndrome
https://orcid.org/0000-0002-3549-7593
https://orcid.org/0000-0001-5934-7816
AbstractKlinefelter syndrome (KS), characterized by the presence of at least one extra X‐chromosome, is a common cause of male infertility. However, the mechanism underlying the failure of germline specification is not well studied. Intriguingly, the differentiation efficiency of female human pluripotent stem cells (hPSCs) is often lower than that of male. This study investigates how X‐linked gene dosage affects human primordial germ cell‐like cells (hPGCLCs) specification in both healthy and diseased conditions. This work reveals that X‐linked genes play a multifaceted role against the fate competency to hPGCLCs, with escape genes IGSF1 and CHRDL1 inhibiting the TGF‐beta/Activin A and BMP pathways, respectively. Notably, this work identifies a previously unrecognized role of SOX2, upregulated by the escape gene USP9X, elucidating a species‐specific function in the mammalian germline. The USP9X‐SOX2 regulatory axis profoundly influenced cellular metabolism, mitochondrial morphology, and progenitor competence in hPGCLCs specification. Furthermore, the inability to downregulate SOX2 and upregulate SOX17 in response to BMP signaling impedes downstream gene activation due to motif binding competition. These findings shed novel insights into the human germline specification by elucidating the divergent roles of SOX2 versus SOX17 in mammals, influenced by X‐linked gene dosage effects. These results offer potential applications for improving the induction efficiency of hPGCLCs, facilitating disease mechanistic studies.
X‐Linked Gene Dosage and SOX2 Act as Key Roadblocks for Human Germ Cell Specification in Klinefelter Syndrome
Advanced Science
He, Wenteng (author) / Luo, Qing (author) / Zhao, Jian (author) / Wang, Mengting (author) / Zhao, Allan (author) / Feng, Luohua (author) / Reda, Ahmed (author) / Lindgren, Eva (author) / Stukenborg, Jan‐Bernd (author) / Chen, Jiayu (author)
2025-02-25
Article (Journal)
Electronic Resource
English
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