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Effect of Wastewater Nitrification/Denitrification Treatment on Biofilm Expansion and Ammonia‐Oxidizing/Denitrifying Community
By considering its importance in biological wastewater treatment systems, biofilm formation over time and under activated sludge inoculation in a fixed biomass reactor was investigated. Experiments were conducted on a reactor composed of two successive PVC columns; a first gravel column and a second sandy one. The physico–chemical and bacteriological quality of treated water at the outlet of the biological reactor was tracked. Furthermore, a quantitative and qualitative study was made on the biofilm before and after addition of a carbon source. Thus, the attached volatile solid (AVS), exopolysaccharides (EPS), proteins (PN), and microbial DNA were quantified. The results showed that in the first column and at 80 and 168 cm depth, AVS and DNA were greater than those registered at 5 cm. A similar result was observed in the second column. Nevertheless, at the second sandy column and after sludge addition, the amounts of EPS and PN recorded at depth of 83 and 165 cm were smaller than those obtained at 5 and 10 cm of depth. On the other hand, the polymerase chain reaction‐denaturing gradient gel electrophoresis method coupled with sequence analysis was used to survey the diversity of ammonia‐oxidizing and denitrifying communities. The amoA gene sequencing showed that the majority of the ammonia‐oxidizing communities was affiliated to Nitrosomonas. With the second column sludge addition, the denitrifying community seems to be affected. A great diversity of denitrifying bacteria in 168 cm of depth was observed.
Effect of Wastewater Nitrification/Denitrification Treatment on Biofilm Expansion and Ammonia‐Oxidizing/Denitrifying Community
By considering its importance in biological wastewater treatment systems, biofilm formation over time and under activated sludge inoculation in a fixed biomass reactor was investigated. Experiments were conducted on a reactor composed of two successive PVC columns; a first gravel column and a second sandy one. The physico–chemical and bacteriological quality of treated water at the outlet of the biological reactor was tracked. Furthermore, a quantitative and qualitative study was made on the biofilm before and after addition of a carbon source. Thus, the attached volatile solid (AVS), exopolysaccharides (EPS), proteins (PN), and microbial DNA were quantified. The results showed that in the first column and at 80 and 168 cm depth, AVS and DNA were greater than those registered at 5 cm. A similar result was observed in the second column. Nevertheless, at the second sandy column and after sludge addition, the amounts of EPS and PN recorded at depth of 83 and 165 cm were smaller than those obtained at 5 and 10 cm of depth. On the other hand, the polymerase chain reaction‐denaturing gradient gel electrophoresis method coupled with sequence analysis was used to survey the diversity of ammonia‐oxidizing and denitrifying communities. The amoA gene sequencing showed that the majority of the ammonia‐oxidizing communities was affiliated to Nitrosomonas. With the second column sludge addition, the denitrifying community seems to be affected. A great diversity of denitrifying bacteria in 168 cm of depth was observed.
Effect of Wastewater Nitrification/Denitrification Treatment on Biofilm Expansion and Ammonia‐Oxidizing/Denitrifying Community
Rajeb, Asma Ben (author) / Mehri, Inès (author) / Nasr, Houda (author) / Hassen, Abdennaceur (author)
CLEAN – Soil, Air, Water ; 43 ; 1295-1306
2015-09-01
13 pages
Article (Journal)
Electronic Resource
English
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