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Rapid Detection of Escherichia coli O157:H7 in Water
Escherichia coli O157 can cause life‐threatening disease in the young and the elderly, and numerous outbreaks have been reported. Biochemical detection procedures are inappropriate for environmental monitoring, and simple, rapid, and sensitive isolation and detection procedures are required for improved surveillance. Four immunological assays (Reveal®, Eclipse™, ImmunoCard STAT!, and the E. coli O157 Antigen Detection Test) and one molecular assay (BAX®) were evaluated to measure sensitivity for E. coli O157:H7 detection in water. Promising procedures were combined with sample concentration, enrichment, and capture using immunomagnetic separation. Method sensitivity was determined using various environmental water matrixes in both single‐ and multilaboratory evaluations to assess method robustness. Overall method sensitivity was ±0.05 cfu/mL using detection by both Reveal and BAX methods, which was considered suitable for environmental screening. The BAX method, because of lower cross‐reactivity, was effective for both raw and finished water analyses whereas the Reveal method was suited for finished water.
Rapid Detection of Escherichia coli O157:H7 in Water
Escherichia coli O157 can cause life‐threatening disease in the young and the elderly, and numerous outbreaks have been reported. Biochemical detection procedures are inappropriate for environmental monitoring, and simple, rapid, and sensitive isolation and detection procedures are required for improved surveillance. Four immunological assays (Reveal®, Eclipse™, ImmunoCard STAT!, and the E. coli O157 Antigen Detection Test) and one molecular assay (BAX®) were evaluated to measure sensitivity for E. coli O157:H7 detection in water. Promising procedures were combined with sample concentration, enrichment, and capture using immunomagnetic separation. Method sensitivity was determined using various environmental water matrixes in both single‐ and multilaboratory evaluations to assess method robustness. Overall method sensitivity was ±0.05 cfu/mL using detection by both Reveal and BAX methods, which was considered suitable for environmental screening. The BAX method, because of lower cross‐reactivity, was effective for both raw and finished water analyses whereas the Reveal method was suited for finished water.
Rapid Detection of Escherichia coli O157:H7 in Water
Bukhari, Zia (author) / Weihe, Janice R. (author) / Lechevallier, Mark W. (author)
Journal ‐ American Water Works Association ; 99 ; 157-167
2007-09-01
11 pages
Article (Journal)
Electronic Resource
English
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