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Toxicology of a Microcystis ichthyoblabe waterbloom from Lake Oued Mellah (Morocco)
10.1002/tox.10028.abs
In the Lake Oued Mellah cyanobacteria waterblooms occur periodically in late spring and summer with Microcystis ichthyoblabe as the main bloom‐forming species. In 1999, a heavy waterbloom of M. ichthyoblabe occurred during May June with a maximal biomass of 298 mg/l. During this period, several bloom samples were collected. The toxicity assessment was done by mouse and brine shrimp (Artemia) bioassays. Apart from the sample collected on 15/06/1999, all the other samples were toxic by mouse bioassay. The LD50 determined by intraperitoneal injection to mice during active cyanobacterial growth and decline phases were 518 and 1924 mgDW/kg respectively. Using Artemia bioassay, the 24hLC50 varied from 6.0 to 40.6 mg/ml and the 48hLC50 ranged from 2.8 to 18.2 mg/ml. The separation and identification of microcystin variants was performed by high performance liquid chromatography–photodiode array detection. Eleven toxins were separated and preliminarily identified as microcystin variants as they exhibit a typical UV spectra like the microcystin‐LR standard. The quantification of total microcystins determined by enzyme‐linked immunosorbent assay showed that the contents were varied between 0.1 and 0.76 μg/g DW. © 2002 by Wiley Periodicals, Inc. Environ Toxicol 17: 24–31, 2002
Toxicology of a Microcystis ichthyoblabe waterbloom from Lake Oued Mellah (Morocco)
10.1002/tox.10028.abs
In the Lake Oued Mellah cyanobacteria waterblooms occur periodically in late spring and summer with Microcystis ichthyoblabe as the main bloom‐forming species. In 1999, a heavy waterbloom of M. ichthyoblabe occurred during May June with a maximal biomass of 298 mg/l. During this period, several bloom samples were collected. The toxicity assessment was done by mouse and brine shrimp (Artemia) bioassays. Apart from the sample collected on 15/06/1999, all the other samples were toxic by mouse bioassay. The LD50 determined by intraperitoneal injection to mice during active cyanobacterial growth and decline phases were 518 and 1924 mgDW/kg respectively. Using Artemia bioassay, the 24hLC50 varied from 6.0 to 40.6 mg/ml and the 48hLC50 ranged from 2.8 to 18.2 mg/ml. The separation and identification of microcystin variants was performed by high performance liquid chromatography–photodiode array detection. Eleven toxins were separated and preliminarily identified as microcystin variants as they exhibit a typical UV spectra like the microcystin‐LR standard. The quantification of total microcystins determined by enzyme‐linked immunosorbent assay showed that the contents were varied between 0.1 and 0.76 μg/g DW. © 2002 by Wiley Periodicals, Inc. Environ Toxicol 17: 24–31, 2002
Toxicology of a Microcystis ichthyoblabe waterbloom from Lake Oued Mellah (Morocco)
Sabour, Brahim (author) / Loudiki, Mohammed (author) / Oudra, Brahim (author) / Vasconcelos, Vitor (author) / Martins, Rosario (author) / Oubraim, Said (author) / Fawzi, Brahim (author)
Environmental Toxicology ; 17 ; 24-31
2002-01-01
8 pages
Article (Journal)
Electronic Resource
English
Toxicology of a Microcystis ichthyoblabe Waterbloom from Lake Oued Mellah (Morocco)
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