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Circ_SNX27 regulates hepatocellular carcinoma development via miR‐637/FGFR1 axis
Background: Circular RNAs (circRNAs) serve as critical regulatory factors in cancer development. Nonetheless, the potential regulatory mechanism of circRNA sorting nexin 27 (circ_SNX27) in hepatocellular carcinoma (HCC) is still unknown.
Methods: The circ_SNX27, microRNA‐637 (miR‐637), and fibroblast growth factor receptor 1 (FGFR1) levels were quantified by quantitative real‐time polymerase chain reaction and western blot analysis. Next, function experiments were conducted using in vitro assays and in vivo senograft study. The relationship between miR‐637 with circ_SNX27 or FGFR1 was uncovered by dual‐luciferase reporter and RNA pull‐down assays.
Results: The circ_SNX27 and FGFR1 levels were up‐regulated, but miR‐637 content was reduced in HCC. Circ_SNX27 down‐regulation inhibited HCC cell proliferation, motility, and invasion and promoted apoptosis in vitro, as well as weakened tumor growth in vivo. Circ_SNX27 served as a sponge of miR‐637 to promote FGFR1 expression. MiR‐637 reduction abolished the restrained effect of circ_SNX27 absence on HCC cell development. Moreover, miR‐637 curbed HCC cell malignant phenotype by regulating FGFR1.
Conclusion: Circ_SNX27 contributed to HCC development via miR‐637/FGFR1 axis, offering a new idea for the treatment of HCC.
Circ_SNX27 regulates hepatocellular carcinoma development via miR‐637/FGFR1 axis
Background: Circular RNAs (circRNAs) serve as critical regulatory factors in cancer development. Nonetheless, the potential regulatory mechanism of circRNA sorting nexin 27 (circ_SNX27) in hepatocellular carcinoma (HCC) is still unknown.
Methods: The circ_SNX27, microRNA‐637 (miR‐637), and fibroblast growth factor receptor 1 (FGFR1) levels were quantified by quantitative real‐time polymerase chain reaction and western blot analysis. Next, function experiments were conducted using in vitro assays and in vivo senograft study. The relationship between miR‐637 with circ_SNX27 or FGFR1 was uncovered by dual‐luciferase reporter and RNA pull‐down assays.
Results: The circ_SNX27 and FGFR1 levels were up‐regulated, but miR‐637 content was reduced in HCC. Circ_SNX27 down‐regulation inhibited HCC cell proliferation, motility, and invasion and promoted apoptosis in vitro, as well as weakened tumor growth in vivo. Circ_SNX27 served as a sponge of miR‐637 to promote FGFR1 expression. MiR‐637 reduction abolished the restrained effect of circ_SNX27 absence on HCC cell development. Moreover, miR‐637 curbed HCC cell malignant phenotype by regulating FGFR1.
Conclusion: Circ_SNX27 contributed to HCC development via miR‐637/FGFR1 axis, offering a new idea for the treatment of HCC.
Circ_SNX27 regulates hepatocellular carcinoma development via miR‐637/FGFR1 axis
Li, Hua (author) / Liu, Bingli (author) / Xu, Xin (author) / Li, Shunle (author) / Zhang, Di (author) / Liu, Qingfeng (author)
Environmental Toxicology ; 37 ; 2832-2843
2022-12-01
12 pages
Article (Journal)
Electronic Resource
English
CSN6‐SPOP‐HMGCS1 Axis Promotes Hepatocellular Carcinoma Progression via YAP1 Activation
| Wiley | 2024
CSN6‐SPOP‐HMGCS1 Axis Promotes Hepatocellular Carcinoma Progression via YAP1 Activation
| Wiley | 2024