A platform for research: civil engineering, architecture and urbanism
A platform for research: civil engineering, architecture and urbanism
Acclimation of 2‐Chlorophenol‐Biodegrading Activated Sludge and Microbial Community Analysis
Using glucose as cosubstrate, activated sludge that could effectively biodegrade 40 mg/L 2‐chlorophenol was successfully domesticated in sequencing batch reactors. To acclimate the sludge, 2‐chlorophenol was increased stepwise from 0 to 40 mg/L. High‐throughput sequencing revealed that the microbial community richness increased during the first 5 d of acclimation to 5 mg/L 2‐chlorophenol and then decreased after another 20 d as 2‐chlorophenol was increased. The original sludge obtained from a water resource recovery facility had the highest microbial diversity. As the acclimation continued further, community richness and diversity both increased, but they decreased again, significantly, when 2‐chlorophenol reached 40 mg/L. Saccharibacteria_norank, Bacillus, Saprospiraceae_uncultured, and Lactococcus were the dominant bacteria. Bacillus and Pseudomonas were the main known chlorophenol‐degrading bacteria. WCHB1‐60_norank, Tetrasphaera, Comamonadaceae_unclassified, and Haliangium showed poor tolerance to 2‐chlorophenol. Higher bacterial tolerance to chlorophenols does not mean higher degrading capability. The degradation of chlorophenols was not positively correlated with the detected abundance of known 2‐chlorophenol‐degrading bacteria.
Acclimation of 2‐Chlorophenol‐Biodegrading Activated Sludge and Microbial Community Analysis
Using glucose as cosubstrate, activated sludge that could effectively biodegrade 40 mg/L 2‐chlorophenol was successfully domesticated in sequencing batch reactors. To acclimate the sludge, 2‐chlorophenol was increased stepwise from 0 to 40 mg/L. High‐throughput sequencing revealed that the microbial community richness increased during the first 5 d of acclimation to 5 mg/L 2‐chlorophenol and then decreased after another 20 d as 2‐chlorophenol was increased. The original sludge obtained from a water resource recovery facility had the highest microbial diversity. As the acclimation continued further, community richness and diversity both increased, but they decreased again, significantly, when 2‐chlorophenol reached 40 mg/L. Saccharibacteria_norank, Bacillus, Saprospiraceae_uncultured, and Lactococcus were the dominant bacteria. Bacillus and Pseudomonas were the main known chlorophenol‐degrading bacteria. WCHB1‐60_norank, Tetrasphaera, Comamonadaceae_unclassified, and Haliangium showed poor tolerance to 2‐chlorophenol. Higher bacterial tolerance to chlorophenols does not mean higher degrading capability. The degradation of chlorophenols was not positively correlated with the detected abundance of known 2‐chlorophenol‐degrading bacteria.
Acclimation of 2‐Chlorophenol‐Biodegrading Activated Sludge and Microbial Community Analysis
Sun, Zhirong (author) / Zhang, Jinwei (author) / Yang, Jie (author) / Li, Jiangyang (author) / Wang, Jianguang (author) / Hu, Xiang (author)
Water Environment Research ; 90 ; 2083-2089
2018-12-01
7 pages
Article (Journal)
Electronic Resource
English
| Taylor & Francis Verlag | 2019
An ecosystem analysis of the activated sludge microbial community
| Taylor & Francis Verlag | 2010
Plasmid Conjugation in an Activated Sludge Microbial Community
| British Library Online Contents | 2009