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Application of microbial assay for risk assessment biotest in evaluation of toxicity of human and veterinary antibiotics
The microbial assay for risk assessment (MARA) is a multispecies, growth inhibition microplate toxicity test with 11 microorganisms individually lyophilized in microplate wells. The microbial species representing wide diversity, generated 11 microbial toxic concentration (MTC) values were obtained giving a unique “toxic fingerprint” profile of the test sample. The toxicity of 14 antibiotics was evaluated with the MARA test. The fingerprints for each group of antibiotics differ, indicating a particular toxic profile. Tetracyclines were the most toxic antibacterials with the minimum MTC value of 3.6 μg L−1. In the group of tetracyclines the order of the three most sensitive microbial strains was the same 2 > 6 > 7. Quinolones affected the most sensitive strain(s) at concentrations of 12–75 μg L−1. The MARA bioassay exhibited different spectra of toxic responses to microbial strains for the first and second generation quinolones. However, for first generation quinolones strain 6 was substantially more sensitive than the other microorganisms, while second generation quinolones were most toxic to strain 3, followed by 6 then 4. In this instance, the three strains belong to two different phylogenetic groups–strain 3 is γ‐proteotype and strains 4 and 6 are β‐proteotype. © 2010 Wiley Periodicals, Inc. Environ Toxicol, 2010.
Application of microbial assay for risk assessment biotest in evaluation of toxicity of human and veterinary antibiotics
The microbial assay for risk assessment (MARA) is a multispecies, growth inhibition microplate toxicity test with 11 microorganisms individually lyophilized in microplate wells. The microbial species representing wide diversity, generated 11 microbial toxic concentration (MTC) values were obtained giving a unique “toxic fingerprint” profile of the test sample. The toxicity of 14 antibiotics was evaluated with the MARA test. The fingerprints for each group of antibiotics differ, indicating a particular toxic profile. Tetracyclines were the most toxic antibacterials with the minimum MTC value of 3.6 μg L−1. In the group of tetracyclines the order of the three most sensitive microbial strains was the same 2 > 6 > 7. Quinolones affected the most sensitive strain(s) at concentrations of 12–75 μg L−1. The MARA bioassay exhibited different spectra of toxic responses to microbial strains for the first and second generation quinolones. However, for first generation quinolones strain 6 was substantially more sensitive than the other microorganisms, while second generation quinolones were most toxic to strain 3, followed by 6 then 4. In this instance, the three strains belong to two different phylogenetic groups–strain 3 is γ‐proteotype and strains 4 and 6 are β‐proteotype. © 2010 Wiley Periodicals, Inc. Environ Toxicol, 2010.
Application of microbial assay for risk assessment biotest in evaluation of toxicity of human and veterinary antibiotics
Nałȩcz‐Jawecki, G. (author) / Wadhia, K. (author) / Adomas, B. (author) / Piotrowicz‐Cieślak, A.I. (author) / Sawicki, J. (author)
Environmental Toxicology ; 25 ; 487-494
2010-10-01
8 pages
Article (Journal)
Electronic Resource
English
MARA , antibiotics , microbial , bioassay
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