A platform for research: civil engineering, architecture and urbanism
A platform for research: civil engineering, architecture and urbanism
4‐chloro‐1,2‐phenylenediamine Induces Apoptosis in Mardin–Darby canine kidney cells via activation of caspases
4‐Chloro‐1,2‐phenylenediamine (4‐Cl‐o‐PD) is a halogenated aromatic diamine that was used as a precursor for manufacturing permanent hair dyes. Despite its well‐documented mutagenic and carcinogenic effects in a number of in vitro and in vivo models, its cytotoxicity and mode of action have not received similar attention. Here, we investigated the effect of 4‐Cl‐o‐PD on Mardin–Darby canine kidney cells. It induced apoptosis and the evidence suggests its initiation by reactive oxygen species (ROS). The results of various assays used show a dose‐dependent (i) decrease in cell viability, (ii) increase in cells at sub‐G1 phase and the G0/G1 phase arrested in cell cycle, (iii) increase in intracellular ROS accompanied by depletion of glutathione, and (iv) that apoptotic cell death probably involves activation of both intrinsic and extrinsic pathways. © 2012 Wiley Periodicals, Inc. Environ Toxicol 29: 655–664, 2014.
4‐chloro‐1,2‐phenylenediamine Induces Apoptosis in Mardin–Darby canine kidney cells via activation of caspases
4‐Chloro‐1,2‐phenylenediamine (4‐Cl‐o‐PD) is a halogenated aromatic diamine that was used as a precursor for manufacturing permanent hair dyes. Despite its well‐documented mutagenic and carcinogenic effects in a number of in vitro and in vivo models, its cytotoxicity and mode of action have not received similar attention. Here, we investigated the effect of 4‐Cl‐o‐PD on Mardin–Darby canine kidney cells. It induced apoptosis and the evidence suggests its initiation by reactive oxygen species (ROS). The results of various assays used show a dose‐dependent (i) decrease in cell viability, (ii) increase in cells at sub‐G1 phase and the G0/G1 phase arrested in cell cycle, (iii) increase in intracellular ROS accompanied by depletion of glutathione, and (iv) that apoptotic cell death probably involves activation of both intrinsic and extrinsic pathways. © 2012 Wiley Periodicals, Inc. Environ Toxicol 29: 655–664, 2014.
4‐chloro‐1,2‐phenylenediamine Induces Apoptosis in Mardin–Darby canine kidney cells via activation of caspases
Onn, Leong Chee (author) / Ching, Chen Ssu (author) / Lian, Tiong Yee (author) / Foon, Loh Veng (author) / Chew Hee, Ng (author) / Moi, Chye Soi (author)
Environmental Toxicology ; 29 ; 655-664
2014-06-01
10 pages
Article (Journal)
Electronic Resource
English
Regeneration of peritoneal effluent by Madin-Darby canine kidney cells-lined hollow fibers
| British Library Online Contents | 1998