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Comparative Study on Mechanistic Kinetic Modeling of the Enzymatic Synthesis of Galacto‐Oligosaccharides
The galacto‐oligosaccharide synthesis by β‐galactosidase from Bacillus circulans is described mathematically using three kinetic models of different complexity – a simple power law approach, an extended Michaelis Menten kinetics and, in particular, an advanced, innovative mechanistic kinetic model for biocatalysis. Based on a detailed experimental study, a comprehensive reaction network is identified, and a reliable database provided. The evaluation of the kinetic models studied is performed by the Akaike information criterion, which allows the assessment of the model accuracy taking into account complexity and transferability.
Comparative Study on Mechanistic Kinetic Modeling of the Enzymatic Synthesis of Galacto‐Oligosaccharides
The galacto‐oligosaccharide synthesis by β‐galactosidase from Bacillus circulans is described mathematically using three kinetic models of different complexity – a simple power law approach, an extended Michaelis Menten kinetics and, in particular, an advanced, innovative mechanistic kinetic model for biocatalysis. Based on a detailed experimental study, a comprehensive reaction network is identified, and a reliable database provided. The evaluation of the kinetic models studied is performed by the Akaike information criterion, which allows the assessment of the model accuracy taking into account complexity and transferability.
Comparative Study on Mechanistic Kinetic Modeling of the Enzymatic Synthesis of Galacto‐Oligosaccharides
Mueller, Ines (author) / Runne, Eike (author) / Hamel, Christof (author)
Chemie Ingenieur Technik ; 94 ; 774-779
2022-05-01
6 pages
Article (Journal)
Electronic Resource
English