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Chaperonin–Dendrimer Conjugates for siRNA Delivery

Nussbaumer, Martin G. / Duskey, Jason T. / Rother, Martin / Renggli, Kasper / Chami, Mohamed / Bruns, Nico

The group II chaperonin thermosome (THS) is a hollow protein nanoparticle that can encapsulate macromolecular guests. Two large pores grant access to the interior of the protein cage. Poly(amidoamine) (PAMAM) is conjugated into THS to act as an anchor for small interfering RNA (siRNA), allowing to load the THS with therapeutic payload. THS–PAMAM protects siRNA from degradation by RNase A and traffics KIF11 and GAPDH siRNA into U87 cancer cells. By modification of the protein cage with the cell‐penetrating peptide TAT, RNA interference is also induced in PC‐3 cells. THS–PAMAM protein–polymer conjugates are therefore promising siRNA transfection reagents and greatly expand the scope of protein cages in drug delivery applications.

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Chaperonin–Dendrimer Conjugates for siRNA Delivery

Nussbaumer, Martin G. / Duskey, Jason T. / Rother, Martin / Renggli, Kasper / Chami, Mohamed / Bruns, Nico

The group II chaperonin thermosome (THS) is a hollow protein nanoparticle that can encapsulate macromolecular guests. Two large pores grant access to the interior of the protein cage. Poly(amidoamine) (PAMAM) is conjugated into THS to act as an anchor for small interfering RNA (siRNA), allowing to load the THS with therapeutic payload. THS–PAMAM protects siRNA from degradation by RNase A and traffics KIF11 and GAPDH siRNA into U87 cancer cells. By modification of the protein cage with the cell‐penetrating peptide TAT, RNA interference is also induced in PC‐3 cells. THS–PAMAM protein–polymer conjugates are therefore promising siRNA transfection reagents and greatly expand the scope of protein cages in drug delivery applications.

Chaperonin–Dendrimer Conjugates for siRNA Delivery

Nussbaumer, Martin G. / Duskey, Jason T. / Rother, Martin / Renggli, Kasper / Chami, Mohamed / Bruns, Nico

The group II chaperonin thermosome (THS) is a hollow protein nanoparticle that can encapsulate macromolecular guests. Two large pores grant access to the interior of the protein cage. Poly(amidoamine) (PAMAM) is conjugated into THS to act as an anchor for small interfering RNA (siRNA), allowing to load the THS with therapeutic payload. THS–PAMAM protects siRNA from degradation by RNase A and traffics KIF11 and GAPDH siRNA into U87 cancer cells. By modification of the protein cage with the cell‐penetrating peptide TAT, RNA interference is also induced in PC‐3 cells. THS–PAMAM protein–polymer conjugates are therefore promising siRNA transfection reagents and greatly expand the scope of protein cages in drug delivery applications.

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Title:

Chaperonin–Dendrimer Conjugates for siRNA Delivery

Contributors:

Nussbaumer, Martin G. (author) / Duskey, Jason T. (author) / Rother, Martin (author) / Renggli, Kasper (author) / Chami, Mohamed (author) / Bruns, Nico (author)

Published in:

Advanced Science ; 3

Publication date:

2016-10-01

Size:

10 pages

ISSN:

DOI:

https://doi.org/10.1002/advs.201600046

Type of media:

Article (Journal)

Type of material:

Electronic Resource

Language:

English

Keywords:

nucleotide delivery vehicle , drug delivery nanosystems , protein cages , RNA interference , small interfering RNA , protein–polymer conjugates

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