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Kinetic Modeling for Simultaneous Biodegradation of Phenol and Formaldehyde at Inhibitory Concentrations by Ralstonia eutropha
The degradation of phenol and formaldehyde (FA) at different concentrations as single or binary systems were experimentally investigated by a pure culture of Ralstonia eutropha in a batch mixed bioreactor operated at 30°C, initial pH 6.5, an aeration rate of 0.5 vvm, and 200 rpm, to determine the kinetics parameters of the processes. In single substrate experiments, the results of the kinetics studies with the Andrews and Noack equation showed that phenol and FA had a self‐inhibitory effect on their biodegradation when the initial concentration was higher than 284.1 and 215.4 mg L−1, respectively. In addition, kinetics studies indicated that partial non‐competitive enzyme inhibition could be used to describe the interactional effects of phenol and FA in the simultaneous biodegradation process. According to the non‐competitive inhibition kinetics applied, the inhibitory constant of phenol on FA biodegradation (KI 1→2) and partiality factor (β1→2) were estimated to be 81.31 mg L−1 and 0.357. In order to describe the effect of FA concentration on the phenol biodegradation, the following results were obtained: KI 2→1 = 37.63 and β2→1 = 0.171 mg L−1. The prediction capacity of the estimated parameters was examined using a model which could describe the dynamic feature of the bioprocesses.
Kinetic Modeling for Simultaneous Biodegradation of Phenol and Formaldehyde at Inhibitory Concentrations by Ralstonia eutropha
The degradation of phenol and formaldehyde (FA) at different concentrations as single or binary systems were experimentally investigated by a pure culture of Ralstonia eutropha in a batch mixed bioreactor operated at 30°C, initial pH 6.5, an aeration rate of 0.5 vvm, and 200 rpm, to determine the kinetics parameters of the processes. In single substrate experiments, the results of the kinetics studies with the Andrews and Noack equation showed that phenol and FA had a self‐inhibitory effect on their biodegradation when the initial concentration was higher than 284.1 and 215.4 mg L−1, respectively. In addition, kinetics studies indicated that partial non‐competitive enzyme inhibition could be used to describe the interactional effects of phenol and FA in the simultaneous biodegradation process. According to the non‐competitive inhibition kinetics applied, the inhibitory constant of phenol on FA biodegradation (KI 1→2) and partiality factor (β1→2) were estimated to be 81.31 mg L−1 and 0.357. In order to describe the effect of FA concentration on the phenol biodegradation, the following results were obtained: KI 2→1 = 37.63 and β2→1 = 0.171 mg L−1. The prediction capacity of the estimated parameters was examined using a model which could describe the dynamic feature of the bioprocesses.
Kinetic Modeling for Simultaneous Biodegradation of Phenol and Formaldehyde at Inhibitory Concentrations by Ralstonia eutropha
Habibi, Alireza (author) / Sharifi, Shahin (author)
CLEAN – Soil, Air, Water ; 44 ; 1113-1122
2016-09-01
11 pages
Article (Journal)
Electronic Resource
English
Formaldehyde degradation by Ralstonia eutropha in an immobilized cell bioreactor
| Online Contents | 2013