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House Dust Induces IL‐6 and IL‐8 Response in A549 Epithelial Cells
Abstract The in vitro potency of house dust to induce cytokine response in A549 lung epithelial cells was studied. Dusts collected from carpet, bed, shelf and floor of a villa and an apartment by vacuuming were found to trigger the production of in‐terleukin–8 (IL–8) and interleukin–6 (IL–6) in a dose–dependent manner, and the interleukin production was several–fold higher than of swine dust (used as a positive control). The IL–8 and IL–6 production of pure Escherichia coli lipopolysaccharide was significantly lower than of the dusts and a peptidoglycan‐polysac‐charide complex did not show any stimulatory effect at all. The lipopolysaccharide and peptidoglycan contents of the samples were determined by gas chromatography–tandem mass spectrometry analysis of, respectively, 3‐hydroxy fatty acids and muramic acid; in addition, ergosterol was monitored for fungal bio‐mass. The inflammatory properties of house dust upon inhalation may be reflected in its high potency to induce cytokine response in lung epithelial cells.
House Dust Induces IL‐6 and IL‐8 Response in A549 Epithelial Cells
Abstract The in vitro potency of house dust to induce cytokine response in A549 lung epithelial cells was studied. Dusts collected from carpet, bed, shelf and floor of a villa and an apartment by vacuuming were found to trigger the production of in‐terleukin–8 (IL–8) and interleukin–6 (IL–6) in a dose–dependent manner, and the interleukin production was several–fold higher than of swine dust (used as a positive control). The IL–8 and IL–6 production of pure Escherichia coli lipopolysaccharide was significantly lower than of the dusts and a peptidoglycan‐polysac‐charide complex did not show any stimulatory effect at all. The lipopolysaccharide and peptidoglycan contents of the samples were determined by gas chromatography–tandem mass spectrometry analysis of, respectively, 3‐hydroxy fatty acids and muramic acid; in addition, ergosterol was monitored for fungal bio‐mass. The inflammatory properties of house dust upon inhalation may be reflected in its high potency to induce cytokine response in lung epithelial cells.
House Dust Induces IL‐6 and IL‐8 Response in A549 Epithelial Cells
Saraf, Anita (author) / Larsson, Lennart (author) / Larsson, Britt‐Marie (author) / Larsson, Kjell (author) / Palmberg, Lena (author)
Indoor Air ; 9 ; 219-225
1999-12-01
7 pages
Article (Journal)
Electronic Resource
English
A549 cells , Biomarkers , GC‐MSMS , IL‐6 , House dust , IL‐8