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Label‐Free Multiphoton Endomicroscopy for Minimally Invasive In Vivo Imaging
Multiphoton microscopy of cellular autofluorescence and second harmonic generation from collagen facilitates imaging of living cells and tissues without the need for additional fluorescent labels. Here, a compact multiphoton endomicroscope for label‐free in vivo imaging in small animals via side‐viewing needle objectives is presented. Minimal invasive imaging at cellular resolution is performed in colonoscopy of mice without surgical measures and without fluorescent dyes as a contrast agent. The colon mucosa is imaged repeatedly in the same animal in a mouse model of acute intestinal inflammation to study the process of inflammation at the tissue level within a time period of ten days, demonstrating the capabilities of label‐free endomicroscopy for longitudinal studies for the first time.
Label‐Free Multiphoton Endomicroscopy for Minimally Invasive In Vivo Imaging
Multiphoton microscopy of cellular autofluorescence and second harmonic generation from collagen facilitates imaging of living cells and tissues without the need for additional fluorescent labels. Here, a compact multiphoton endomicroscope for label‐free in vivo imaging in small animals via side‐viewing needle objectives is presented. Minimal invasive imaging at cellular resolution is performed in colonoscopy of mice without surgical measures and without fluorescent dyes as a contrast agent. The colon mucosa is imaged repeatedly in the same animal in a mouse model of acute intestinal inflammation to study the process of inflammation at the tissue level within a time period of ten days, demonstrating the capabilities of label‐free endomicroscopy for longitudinal studies for the first time.
Label‐Free Multiphoton Endomicroscopy for Minimally Invasive In Vivo Imaging
Dilipkumar, Ashwathama (author) / Al‐Shemmary, Alaa (author) / Kreiß, Lucas (author) / Cvecek, Kristian (author) / Carlé, Birgitta (author) / Knieling, Ferdinand (author) / Gonzales Menezes, Jean (author) / Thoma, Oana‐Maria (author) / Schmidt, Michael (author) / Neurath, Markus F. (author)
Advanced Science ; 6
2019-04-01
8 pages
Article (Journal)
Electronic Resource
English
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