A platform for research: civil engineering, architecture and urbanism
A platform for research: civil engineering, architecture and urbanism
De‐nitrosylation Coordinates Appressorium Function for Infection of the Rice Blast Fungus
As a signaling molecule, nitric oxide (NO) regulates the development and stress response in different organisms. The major biological activity of NO is protein S‐nitrosylation, whose function in fungi remains largely unclear. Here, it is found in the rice blast fungus Magnaporthe oryzae, de‐nitrosylation process is essential for functional appressorium formation during infection. Nitrosative stress caused by excessive accumulation of NO is harmful for fungal infection. While the S‐nitrosoglutathione reductase GSNOR‐mediated de‐nitrosylation removes excess NO toxicity during appressorium formation to promote infection. Through an indoTMT switch labeling proteomics technique, 741 S‐nitrosylation sites in 483 proteins are identified. Key appressorial proteins, such as Mgb1, MagB, Sps1, Cdc42, and septins, are activated by GSNOR through de‐nitrosylation. Removing S‐nitrosylation sites of above proteins is essential for proper protein structure and appressorial function. Therefore, GSNOR‐mediated de‐nitrosylation is an essential regulator for appressorium formation. It is also shown that breaking NO homeostasis by NO donors, NO scavengers, as well as chemical inhibitor of GSNOR, shall be effective methods for fungal disease control.
De‐nitrosylation Coordinates Appressorium Function for Infection of the Rice Blast Fungus
As a signaling molecule, nitric oxide (NO) regulates the development and stress response in different organisms. The major biological activity of NO is protein S‐nitrosylation, whose function in fungi remains largely unclear. Here, it is found in the rice blast fungus Magnaporthe oryzae, de‐nitrosylation process is essential for functional appressorium formation during infection. Nitrosative stress caused by excessive accumulation of NO is harmful for fungal infection. While the S‐nitrosoglutathione reductase GSNOR‐mediated de‐nitrosylation removes excess NO toxicity during appressorium formation to promote infection. Through an indoTMT switch labeling proteomics technique, 741 S‐nitrosylation sites in 483 proteins are identified. Key appressorial proteins, such as Mgb1, MagB, Sps1, Cdc42, and septins, are activated by GSNOR through de‐nitrosylation. Removing S‐nitrosylation sites of above proteins is essential for proper protein structure and appressorial function. Therefore, GSNOR‐mediated de‐nitrosylation is an essential regulator for appressorium formation. It is also shown that breaking NO homeostasis by NO donors, NO scavengers, as well as chemical inhibitor of GSNOR, shall be effective methods for fungal disease control.
De‐nitrosylation Coordinates Appressorium Function for Infection of the Rice Blast Fungus
Hu, Hong (author) / He, Wenhui (author) / Qu, Zhiguang (author) / Dong, Xiang (author) / Ren, Zhiyong (author) / Qin, Mengyuan (author) / Liu, Hao (author) / Zheng, Lu (author) / Huang, Junbin (author) / Chen, Xiao‐Lin (author)
Advanced Science ; 11
2024-07-01
16 pages
Article (Journal)
Electronic Resource
English
De‐nitrosylation Coordinates Appressorium Function for Infection of the Rice Blast Fungus
| Wiley | 2024
Computing geodetic coordinates from geocentric coordinates
| Online Contents | 2004
Computing geodetic coordinates from geocentric coordinates
| Online Contents | 2004
The role S‐nitrosylation in manganese‐induced autophagy dysregulation in SH‐SY5Y cells
| Online Contents | 2017
Study of Dynamic Coordinates Time Function in Mine Subsidence
| British Library Conference Proceedings | 2012