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Oxidative stress in the clam Ruditapes decussatus (Linnaeus, 1758) in relation to polycyclic aromatic hydrocarbon body burden
10.1002/tox.20257.abs
Seasonal variation of antioxidant enzymes activities (superoxide dismutase, catalase, and glutathione peroxidases) and lipid peroxidation (LPO) were studied in the clam Ruditapes decussatus in relation to body burdens of polycyclic aromatic hydrocarbons (PAHs). Clams were sampled in eight sites from the Ria Formosa lagoon. PAH concentrations were seasonally rather than spatially dependent, being higher in summer (August). Antioxidant enzymes activities and LPO levels in the clam digestive gland were also seasonally dependent. Antioxidant enzymes presented distinct seasonal variations: Mit SOD (superoxide dismutase activity measured in the mitochondrial fraction) was induced in the summer and down‐regulated in winter and spring, while Cyt SOD activity (measured in the cytosolic fraction) was highest in autumn and lower in the summer. Neither Mit nor Cyt SOD were related to the clam PAH body burden, suggesting that cells are using other antioxidant systems to eliminate oxyradicals. Catalase (CAT), however, was induced in spring and down‐regulated in summer, the inverse of the PAH concentrations in clam tissues. CAT induction in spring appears to be related to the excess of oxyradicals arising from the metabolic activity associated with the reproductive cycle. Conversely, the decrease in CAT activity in the summer may be related to the high water temperatures reached in the Ria Formosa (up to 30 °C). Glutathione peroxidases (total fraction ‐ T‐GPx and dependent on selenium ‐ Se‐GPx) presented a similar seasonal pattern, and were negatively related to PAH concentrations, which may indicate a precarious state of the clams, associated with PAH toxicity. Similarly, LPO was also inversely correlated to the PAH concentrations indicating that increases in PAH concentrations were not causing membrane oxidative damage in R. decussatus digestive gland. The results suggest that antioxidant enzymes in R. decussatus digestive gland are strongly affected by seasonal factors stressing the need of other experiments to clarify the PAHs effect on this clam species. © 2007 Wiley Periodicals, Inc. Environ Toxicol 22: 203–221, 2007.
Oxidative stress in the clam Ruditapes decussatus (Linnaeus, 1758) in relation to polycyclic aromatic hydrocarbon body burden
10.1002/tox.20257.abs
Seasonal variation of antioxidant enzymes activities (superoxide dismutase, catalase, and glutathione peroxidases) and lipid peroxidation (LPO) were studied in the clam Ruditapes decussatus in relation to body burdens of polycyclic aromatic hydrocarbons (PAHs). Clams were sampled in eight sites from the Ria Formosa lagoon. PAH concentrations were seasonally rather than spatially dependent, being higher in summer (August). Antioxidant enzymes activities and LPO levels in the clam digestive gland were also seasonally dependent. Antioxidant enzymes presented distinct seasonal variations: Mit SOD (superoxide dismutase activity measured in the mitochondrial fraction) was induced in the summer and down‐regulated in winter and spring, while Cyt SOD activity (measured in the cytosolic fraction) was highest in autumn and lower in the summer. Neither Mit nor Cyt SOD were related to the clam PAH body burden, suggesting that cells are using other antioxidant systems to eliminate oxyradicals. Catalase (CAT), however, was induced in spring and down‐regulated in summer, the inverse of the PAH concentrations in clam tissues. CAT induction in spring appears to be related to the excess of oxyradicals arising from the metabolic activity associated with the reproductive cycle. Conversely, the decrease in CAT activity in the summer may be related to the high water temperatures reached in the Ria Formosa (up to 30 °C). Glutathione peroxidases (total fraction ‐ T‐GPx and dependent on selenium ‐ Se‐GPx) presented a similar seasonal pattern, and were negatively related to PAH concentrations, which may indicate a precarious state of the clams, associated with PAH toxicity. Similarly, LPO was also inversely correlated to the PAH concentrations indicating that increases in PAH concentrations were not causing membrane oxidative damage in R. decussatus digestive gland. The results suggest that antioxidant enzymes in R. decussatus digestive gland are strongly affected by seasonal factors stressing the need of other experiments to clarify the PAHs effect on this clam species. © 2007 Wiley Periodicals, Inc. Environ Toxicol 22: 203–221, 2007.
Oxidative stress in the clam Ruditapes decussatus (Linnaeus, 1758) in relation to polycyclic aromatic hydrocarbon body burden
Barreira, Luísa A. (author) / Mudge, Stephen M. (author) / Bebianno, Maria J. (author)
Environmental Toxicology ; 22 ; 203-221
2007-04-01
19 pages
Article (Journal)
Electronic Resource
English
Energy balance of the clam Ruditapes decussatus (Linnaeus, 1758)
| British Library Conference Proceedings | 2002
| British Library Conference Proceedings | 2002
Effects of food rations on gonadal development of the clam Ruditapes decussatus (L., 1758)
| British Library Conference Proceedings | 2002