A platform for research: civil engineering, architecture and urbanism
A platform for research: civil engineering, architecture and urbanism
Phytoremediation Potential of Hemp (Cannabis sativa L.): Identification and Characterization of Heavy Metals Responsive Genes
Soil pollution caused by heavy metals is one of the major problems throughout the world. To maintain a safe and healthy environment for human beings, there is a dire need to identify hyperaccumulator plants and the underlying genes involved in heavy metals stress tolerance and accumulation. The goal of this research is to explore the potential of hemp as a decontaminator of heavy metals by identifying the two important heavy metals responsive genes, glutathione‐disulfidereductase (GSR) and phospholipase D‐α (PLDα). The results revealed heavy metals accumulation; Cu (1530 mg kg−1), Cd (151 mg kg−1), and Ni (123 mg kg−1) in hemp plants’ leaves collected from the contaminated site. This shows the ability of the hemp plant to tolerate heavy metals, perhaps due to the presence of stress tolerance genes. In this study, partial sequences of putative GSR (215 bp) and PLDα (517 bp) genes were identified, responsive to heavy metals stress in hemp leaves. Both genes exhibited 40–60% sequence identity to previously reported genes from other plant species. Glutathione binding residues and conserved arginine residues were found identical in a putative GSR gene to those of other plant species, while the phospholipids binding domain and catalytic domain were found in the PLDα gene. These results will help to improve our understanding about the phytoremediation potential of hemp as well as in manipulating GSR and PLDα genes in breeding programs to produce transgenic heavy‐metals‐tolerant varieties.
Phytoremediation Potential of Hemp (Cannabis sativa L.): Identification and Characterization of Heavy Metals Responsive Genes
Soil pollution caused by heavy metals is one of the major problems throughout the world. To maintain a safe and healthy environment for human beings, there is a dire need to identify hyperaccumulator plants and the underlying genes involved in heavy metals stress tolerance and accumulation. The goal of this research is to explore the potential of hemp as a decontaminator of heavy metals by identifying the two important heavy metals responsive genes, glutathione‐disulfidereductase (GSR) and phospholipase D‐α (PLDα). The results revealed heavy metals accumulation; Cu (1530 mg kg−1), Cd (151 mg kg−1), and Ni (123 mg kg−1) in hemp plants’ leaves collected from the contaminated site. This shows the ability of the hemp plant to tolerate heavy metals, perhaps due to the presence of stress tolerance genes. In this study, partial sequences of putative GSR (215 bp) and PLDα (517 bp) genes were identified, responsive to heavy metals stress in hemp leaves. Both genes exhibited 40–60% sequence identity to previously reported genes from other plant species. Glutathione binding residues and conserved arginine residues were found identical in a putative GSR gene to those of other plant species, while the phospholipids binding domain and catalytic domain were found in the PLDα gene. These results will help to improve our understanding about the phytoremediation potential of hemp as well as in manipulating GSR and PLDα genes in breeding programs to produce transgenic heavy‐metals‐tolerant varieties.
Phytoremediation Potential of Hemp (Cannabis sativa L.): Identification and Characterization of Heavy Metals Responsive Genes
Ahmad, Rafiq (author) / Tehsin, Zara (author) / Malik, Samina Tanvir (author) / Asad, Saeed Ahmad (author) / Shahzad, Muhammad (author) / Bilal, Muhammad (author) / Shah, Mohammad Maroof (author) / Khan, Sabaz Ali (author)
CLEAN – Soil, Air, Water ; 44 ; 195-201
2016-02-01
8 pages
Article (Journal)
Electronic Resource
English
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