A platform for research: civil engineering, architecture and urbanism
A platform for research: civil engineering, architecture and urbanism
Optimization of Ethoxyresorufin‐O‐deethylase Determination in the Microsomes of Earthworms and Its Induction by Polycyclic Aromatic Hydrocarbons
In this study, a fluorometric method was established to measure the ethoxyresorufin‐O‐dealkylase (EROD) activity in the microsomes of earthworm after a series of parameter modification (including excitation wavelengths; kinds, pH, and volumes of buffer solutions; kinds and volumes of sensitizers). Earthworms were exposed to increasing concentrations of benzo[a]pyrene (B[a]P) (0.009–90 µg cm−2) in filter papers to validate the modified method and to find out the possible effects of B[a]P to EROD activity. Results showed that 560 nm was the optimal excitation wavelength for detecting resorufin. The linear range was 0.05–20 pmol mL−1 with the correlation coefficient of 0.9996. The detection limit was 0.045 pmol mL−1 and the relative standard deviation was <1.3%. Based on the established method, we also found that EROD activity in earthworms was significantly induced by B[a]P at 0.009 and 0.09 µg cm−2, suggesting that the EROD activity in earthworms could be served as a sensitive biomarker in the monitoring of soil contamination within low dosage range.
Optimization of Ethoxyresorufin‐O‐deethylase Determination in the Microsomes of Earthworms and Its Induction by Polycyclic Aromatic Hydrocarbons
In this study, a fluorometric method was established to measure the ethoxyresorufin‐O‐dealkylase (EROD) activity in the microsomes of earthworm after a series of parameter modification (including excitation wavelengths; kinds, pH, and volumes of buffer solutions; kinds and volumes of sensitizers). Earthworms were exposed to increasing concentrations of benzo[a]pyrene (B[a]P) (0.009–90 µg cm−2) in filter papers to validate the modified method and to find out the possible effects of B[a]P to EROD activity. Results showed that 560 nm was the optimal excitation wavelength for detecting resorufin. The linear range was 0.05–20 pmol mL−1 with the correlation coefficient of 0.9996. The detection limit was 0.045 pmol mL−1 and the relative standard deviation was <1.3%. Based on the established method, we also found that EROD activity in earthworms was significantly induced by B[a]P at 0.009 and 0.09 µg cm−2, suggesting that the EROD activity in earthworms could be served as a sensitive biomarker in the monitoring of soil contamination within low dosage range.
Optimization of Ethoxyresorufin‐O‐deethylase Determination in the Microsomes of Earthworms and Its Induction by Polycyclic Aromatic Hydrocarbons
Cao, Xiufeng (author) / Song, Yufang (author) / Fan, Shihua (author) / Kai, Jianrong (author) / Yang, Xiaoxia (author) / Chen, Lang (author)
CLEAN – Soil, Air, Water ; 42 ; 1121-1125
2014-08-01
5 pages
Article (Journal)
Electronic Resource
English