A platform for research: civil engineering, architecture and urbanism
A platform for research: civil engineering, architecture and urbanism
Programmable High‐Speed and Hyper‐Efficiency DNA Signal Magnifier
Herein, a programmable dual‐catalyst hairpin assembly (DCHA) for realizing the synchronous recycle of two catalysts is developed, displaying high reaction rate and outstanding conversion efficiency beyond traditional nucleic acid signal amplifications (NASA). Once catalyst I interacts with the catalyst II, the DCHA can be triggered to realize the simultaneous recycle of catalysts I and II to keep the highly concentrated intermediate product duplex I‐II instead of the steadily decreased one in typical NASA, which can accomplish in about only 16 min and achieves the outstanding conversion efficiency up to 4.54 × 108, easily conquering the main predicaments of NASA: time‐consuming and low‐efficiency. As a proof of the concept, the proposed DCHA as a high‐speed and hyper‐efficiency DNA signal magnifier is successfully applied in the rapid and ultrasensitive detection of miRNA‐21 in cancer cell lysates, which exploits the new generation of universal strategy for the applications in biosensing assay, clinic diagnose, and DNA nanobiotechnology.
Programmable High‐Speed and Hyper‐Efficiency DNA Signal Magnifier
Herein, a programmable dual‐catalyst hairpin assembly (DCHA) for realizing the synchronous recycle of two catalysts is developed, displaying high reaction rate and outstanding conversion efficiency beyond traditional nucleic acid signal amplifications (NASA). Once catalyst I interacts with the catalyst II, the DCHA can be triggered to realize the simultaneous recycle of catalysts I and II to keep the highly concentrated intermediate product duplex I‐II instead of the steadily decreased one in typical NASA, which can accomplish in about only 16 min and achieves the outstanding conversion efficiency up to 4.54 × 108, easily conquering the main predicaments of NASA: time‐consuming and low‐efficiency. As a proof of the concept, the proposed DCHA as a high‐speed and hyper‐efficiency DNA signal magnifier is successfully applied in the rapid and ultrasensitive detection of miRNA‐21 in cancer cell lysates, which exploits the new generation of universal strategy for the applications in biosensing assay, clinic diagnose, and DNA nanobiotechnology.
Programmable High‐Speed and Hyper‐Efficiency DNA Signal Magnifier
Zhang, Xiao‐Long (author) / Yin, Yang (author) / Du, Shu‐Min (author) / Kong, Ling‐Qi (author) / Yang, Zhe‐Han (author) / Chang, Yuan‐Yuan (author) / Chai, Ya‐Qin (author) / Yuan, Ruo (author)
Advanced Science ; 9
2022-02-01
9 pages
Article (Journal)
Electronic Resource
English
Biaxial bending moment magnifier method
| Online Contents | 2004
Biaxial bending moment magnifier method
| Elsevier | 2004
Energy Harvester with a Dynamic Magnifier
| British Library Online Contents | 2011
An efficient vibration energy harvester with a multi-mode dynamic magnifier
| British Library Online Contents | 2012
Moment Magnifier Method for Long-Term Behavior of Flat Plates Subjected to In-Plane Compression
| Online Contents | 2003