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False Positive Fecal Coliform in Biosolid Samples Assayed using A‐1 Medium
Two most probable number (MPN) methods—lauryl tryptose broth with Escherichia coli broth confirmation and direct A‐1 broth incubation (A‐1)—were compared for the enumeration of fecal coliform in lime‐treated biosolid. Fecal coliform numbers were significantly higher using the A‐1 method. Analysis of positive A‐1 tubes, however, indicated that a high percentage of these were false positives. Therefore, the use of A‐1 broth for 40 CFR Part 503 Pathogen Reduction (CFR, 1993) compliance testing is not recommended.
False Positive Fecal Coliform in Biosolid Samples Assayed using A‐1 Medium
Two most probable number (MPN) methods—lauryl tryptose broth with Escherichia coli broth confirmation and direct A‐1 broth incubation (A‐1)—were compared for the enumeration of fecal coliform in lime‐treated biosolid. Fecal coliform numbers were significantly higher using the A‐1 method. Analysis of positive A‐1 tubes, however, indicated that a high percentage of these were false positives. Therefore, the use of A‐1 broth for 40 CFR Part 503 Pathogen Reduction (CFR, 1993) compliance testing is not recommended.
False Positive Fecal Coliform in Biosolid Samples Assayed using A‐1 Medium
Baker, Katherine H. (author) / Redmond, Brady (author) / Herson, Diane S. (author)
Water Environment Research ; 77 ; 423-424
2005-07-01
2 pages
Article (Journal)
Electronic Resource
English
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