A platform for research: civil engineering, architecture and urbanism
A platform for research: civil engineering, architecture and urbanism
StretchView – A Multi‐Axial Cell‐Stretching Device for Long‐Term Automated Videomicroscopy of Living Cells
Incorporating mechanical stretching of cells in tissue culture is crucial for mimicking (patho)‐physiological conditions and understanding the mechanobiological responses of cells, which can have significant implications in areas like tissue engineering and regenerative medicine. Despite the growing interest, most available cell‐stretching devices are not compatible with automated live‐cell imaging, indispensable for characterizing alterations in the dynamics of various important cellular processes. In this work, StretchView is presented, a multi‐axial cell‐stretching platform compatible with automated, time‐resolved live‐cell imaging. Using StretchView, long‐term image acquisition of cells in the relaxed and stretched states is shown for the first time (experimental time of 12 h) without the need for human intervention. Homogeneous and stable strain fields are demonstrated for 18 h of cyclic stretching, highlighting the platform's versatility and robustness. As proof‐of‐principle, the effect of stretching on cell kinematics and spatiotemporal localization of the cell‐cell adhesion protein E‐cadherin is examined for MDCK cells in monolayer. First evidence of a monotonic increase in junctional E‐cadherin localization upon exposure to stretch is presented using live‐cell imaging data acquired during cyclic stretching, suggestive of an increase in barrier integrity of the monolayer. These findings highlight the potential of StretchView in providing insights into cell mechanobiology and beyond.
StretchView – A Multi‐Axial Cell‐Stretching Device for Long‐Term Automated Videomicroscopy of Living Cells
Incorporating mechanical stretching of cells in tissue culture is crucial for mimicking (patho)‐physiological conditions and understanding the mechanobiological responses of cells, which can have significant implications in areas like tissue engineering and regenerative medicine. Despite the growing interest, most available cell‐stretching devices are not compatible with automated live‐cell imaging, indispensable for characterizing alterations in the dynamics of various important cellular processes. In this work, StretchView is presented, a multi‐axial cell‐stretching platform compatible with automated, time‐resolved live‐cell imaging. Using StretchView, long‐term image acquisition of cells in the relaxed and stretched states is shown for the first time (experimental time of 12 h) without the need for human intervention. Homogeneous and stable strain fields are demonstrated for 18 h of cyclic stretching, highlighting the platform's versatility and robustness. As proof‐of‐principle, the effect of stretching on cell kinematics and spatiotemporal localization of the cell‐cell adhesion protein E‐cadherin is examined for MDCK cells in monolayer. First evidence of a monotonic increase in junctional E‐cadherin localization upon exposure to stretch is presented using live‐cell imaging data acquired during cyclic stretching, suggestive of an increase in barrier integrity of the monolayer. These findings highlight the potential of StretchView in providing insights into cell mechanobiology and beyond.
StretchView – A Multi‐Axial Cell‐Stretching Device for Long‐Term Automated Videomicroscopy of Living Cells
Jaworski, David (author) / Hundsdorfer, Lara (author) / Bastounis, Effie (author) / Constantinou, Iordania (author)
Advanced Science ; 12
2025-03-01
19 pages
Article (Journal)
Electronic Resource
English
| Wiley | 2025
Direct in vivo Visualization of Renal Microcirculation by Intravital CCD Videomicroscopy
| British Library Online Contents | 2001
Anti-stretching steel support shaft axial pressure device and axial force stepless adjusting method
| European Patent Office | 2020
Conditions for Osteoblast Arrangement Induced under Long-Term Cyclic Stretching
| British Library Online Contents | 2013
Automated Simulation of Metal Stretching Tests
| British Library Online Contents | 1992