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Development of a free β‐galactosidase in vitro test for the assessment of heavy metal toxicity
10.1002/tox.1014.abs
The effect of heavy metals and organic compounds on the activity of the enzyme β‐galactosidase in a standardized bioassay has been evaluated, considering future applications in environmental monitoring. The tests were done using a commercial extract of a hydrolase from the eukaryote yeast Kluyveromyces lactis and o‐nitrophenyl‐β‐D‐galactopyranoside (ONPG) as substrate. The enzyme was exposed to Cr(VI), Cd(II), Cu(II), Ni(II), Pb(II), Hg(II), phenol, sodium dodecyl sulfate, methanol and pentachlorophenol for 5, 15, 30, and 60 min. According to the results, a 15 min exposure time was considered optimum for the performance of the assay. Results of tests with metals showed IC50 values ranging between 9.25mg/L for Cd(II) and 0.015mg/L for Hg(II), with an order of sensitivity of: Cd(II)in vitro free enzyme test showed a similar behavior to other tests based on β‐galactosidase such as the MetPlate™. Furthermore, when compared to data from the literature on acute toxicity assays currently used in environmental assessment, test results show good agreement regarding the sensitivity to metals. After standardization, the proposed test could be used as a rapid and low‐cost assay when evaluating biological effects of heavy metals in monitoring programs. © 2001 John Wiley & Sons, Inc. Environ Toxicol 16: 117–120, 2001
Development of a free β‐galactosidase in vitro test for the assessment of heavy metal toxicity
10.1002/tox.1014.abs
The effect of heavy metals and organic compounds on the activity of the enzyme β‐galactosidase in a standardized bioassay has been evaluated, considering future applications in environmental monitoring. The tests were done using a commercial extract of a hydrolase from the eukaryote yeast Kluyveromyces lactis and o‐nitrophenyl‐β‐D‐galactopyranoside (ONPG) as substrate. The enzyme was exposed to Cr(VI), Cd(II), Cu(II), Ni(II), Pb(II), Hg(II), phenol, sodium dodecyl sulfate, methanol and pentachlorophenol for 5, 15, 30, and 60 min. According to the results, a 15 min exposure time was considered optimum for the performance of the assay. Results of tests with metals showed IC50 values ranging between 9.25mg/L for Cd(II) and 0.015mg/L for Hg(II), with an order of sensitivity of: Cd(II)in vitro free enzyme test showed a similar behavior to other tests based on β‐galactosidase such as the MetPlate™. Furthermore, when compared to data from the literature on acute toxicity assays currently used in environmental assessment, test results show good agreement regarding the sensitivity to metals. After standardization, the proposed test could be used as a rapid and low‐cost assay when evaluating biological effects of heavy metals in monitoring programs. © 2001 John Wiley & Sons, Inc. Environ Toxicol 16: 117–120, 2001
Development of a free β‐galactosidase in vitro test for the assessment of heavy metal toxicity
Apartin, Carina (author) / Ronco, Alicia (author)
Environmental Toxicology ; 16 ; 117-120
2001-01-01
4 pages
Article (Journal)
Electronic Resource
English
Development of a Free b-Galactosidase In Vitro Test for the Assessment of Heavy Metal Toxicity
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